Stiffness of aortic arch and carotid arteries increases in ApoE-knockout mice with high-fat diet: evidence from echocardiography.
Stiffness of aortic arch and carotid arteries increases in ApoE-knockout mice with high-fat diet: evidence from echocardiography.
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发表时间：
2021
影响因子：
2.2
通讯作者：
Ming Tang;L. Hong;Haibin Li;Wanshi Chen;Leon M. Tai;R. Minshall;Wei Huang;Jiwang Chen
中科院分区：
文献类型：
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作者：
Ming Tang;L. Hong;Haibin Li;Wanshi Chen;Leon M. Tai;R. Minshall;Wei Huang;Jiwang Chen
动脉僵硬是动脉粥样硬化的有效预测指标。测量脉搏波速度(PWV)是研究动脉僵硬的金标准方法。本研究旨在通过超声心动图检查动脉硬化早期的动脉僵硬和心功能。采用高脂饮食(HFD)喂养的载脂蛋白E基因敲除(ApoE-/-)小鼠动脉粥样硬化模型，以普通饲料(ND)为对照。超声心动图测定主动脉弓、颈动脉僵硬度及左心室收缩/舒张期功能。测定大鼠血浆胆固醇水平和动脉粥样硬化斑块面积。比较2周、4周、6周和8周不同饮食条件下大鼠主动脉弓和颈动脉的PWV值。8周后，与ND组相比，HFD组小鼠主动脉弓和颈动脉PWV值显著升高(主动脉弓：516.65±216.89 cm/S比192.53±71.71 cm/S；颈动脉：514.26±211.01 cm/S比188.03±75.14 cm/S；P均<0.01)，并伴有左心室收缩/舒张期功能下降。这与血浆胆固醇水平的升高有很好的相关性。在ApoE-/-小鼠中，基于回声的PWV测量在预测动脉粥样硬化方面比在ApoE-/-小鼠的颈动脉更敏感。超声心动图测量主动脉弓PWV可作为动脉粥样硬化早期诊断的一种新方法。
Arterial stiffness is an effective predictor of atherosclerosis. Measurement of pulse-wave velocity (PWV) is a gold-standard approach to study arterial stiffness. This study aims to examine arterial stiffness and heart functions via echocardiography at an early stage of atherosclerosis. A model of atherosclerosis in ApoE-knockout (ApoE-/- ) mice fed on high-fat diet (HFD) was used, with normal chow diet (ND) as a control. Stiffness of aortic arch and carotid arteries and left ventricular (LV) systolic/diastolic functions were measured by echocardiography. The plasma cholesterol levels and atherosclerotic plaque areas in the aortas were measured. The PWV values of aortic arch and carotid arteries were compared at 2, 4, 6 and 8 weeks with different diets. Compared with ND mice, PWV values in aortic arch and carotid arteries were significantly increased in HFD mice after 8 weeks (Aortic arch: 516.65 ± 216.89 cm/s vs. 192.53 ± 71.71 cm/s; Carotid arteries: 514.26 ± 211.01 cm/s vs. 188.03 ± 75.14 cm/s, respectively; both P < 0.01) accompanied by the decrease in LV systolic/diastolic functions. These were well correlated with the increase in plasma cholesterol levels. Echo-based PWV measurement in the aortic arch was found more sensitive to predict atherosclerosis than in the carotid arteries in ApoE-/- mice. Measuring aortic arch PWV via echocardiography could represent a new diagnostic strategy for early detection of atherosclerosis.