Vancomycin-modified Fe(3)O(4)@SiO(2)@Ag microflowers as effective antimicrobial agents.
Vancomycin-modified Fe(3)O(4)@SiO(2)@Ag microflowers as effective antimicrobial agents.
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DOI：
10.2147/ijn.s132570
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发表时间：
2017
影响因子：
8
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Wang C;Zhang K;Zhou Z;Li Q;Shao L;Hao RZ;Xiao R;Wang S
纳米材料与抗生素结合具有协同效应，作为有前途的抗菌剂越来越受到关注。本研究通过合理设计和制备万古霉素修饰磁性银微花（货车/Fe3O4@SiO2@Ag微花），使其具有杀菌能力强、抗菌谱广、可回收性好等特点。高性能的Fe3O4@SiO2@Ag微花作为一个多功能的支持矩阵，并表现出足够的磁响应性能，由于其200 nm的Fe 3 O 4核心。微花还具有高度分支的花状银壳，其提供了大的表面积用于有效的银离子释放和细菌接触。万古霉素修饰层与细菌细胞壁有效结合，增加细胞膜的通透性，促进银离子进入细菌，导致细胞死亡。因此，所制备的货车/Fe3O4@SiO2@Ag微花有望成为一种高效、环保的抗菌剂。这一假设通过对革兰氏阴性大肠杆菌和革兰氏阳性耐甲氧西林金黄色葡萄球菌的灭菌得到验证，最小抑菌浓度分别为10和20 μg mL−1。与裸Fe3O4@SiO2@Ag微花和游离形式的万古霉素相比，微花还显示出增强的效果，证实了两种组分的组合的协同效应。此外，在五次循环试验后，抗菌效果保持在90%以上，表明产品的高稳定性。这些结果表明，货车/Fe3O4@SiO2@Ag微米花在抗菌领域具有广阔的应用前景。
Nanomaterials combined with antibiotics exhibit synergistic effects and have gained increasing interest as promising antimicrobial agents. In this study, vancomycin-modified magnetic-based silver microflowers (Van/Fe3O4@SiO2@Ag microflowers) were rationally designed and prepared to achieve strong bactericidal ability, a wide antimicrobial spectrum, and good recyclability. High-performance Fe3O4@SiO2@Ag microflowers served as a multifunction-supporting matrix and exhibited sufficient magnetic response property due to their 200 nm Fe3O4 core. The microflowers also possessed a highly branched flower-like Ag shell that provided a large surface area for effective Ag ion release and bacterial contact. The modified-vancomycin layer was effectively bound to the cell wall of bacteria to increase the permeability of the cell membrane and facilitate the entry of the Ag ions into the bacterium, resulting in cell death. As such, the fabricated Van/Fe3O4@SiO2@Ag microflowers were predicted to be an effective and environment-friendly antibacterial agent. This hypothesis was verified through sterilization of Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus, with minimum inhibitory concentrations of 10 and 20 μg mL−1, respectively. The microflowers also showed enhanced effect compared with bare Fe3O4@SiO2@Ag microflowers and free-form vancomycin, confirming the synergistic effects of the combination of the two components. Moreover, the antimicrobial effect was maintained at more than 90% after five cycling assays, indicating the high stability of the product. These findings reveal that Van/Fe3O4@SiO2@Ag microflowers exhibit promising applications in the antibacterial fields.