Estimations of the efficacy and reliability of paternity assignments from DNA microsatellite analysis of multiple-sire matings.
Estimations of the efficacy and reliability of paternity assignments from DNA microsatellite analysis of multiple-sire matings.
复制标题
通过多父系交配的 DNA 微卫星分析来估计亲子鉴定的有效性和可靠性。
DOI：
10.1046/j.1365-2052.1999.00511.x
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发表时间：
1999
期刊：
影响因子：
2.4
通讯作者：
M J Faddy
中科院分区：
文献类型：
--
作者：
D M Vankan;M J Faddy
牛DNA检测实验室必须向养牛业提供所提供的DNA检测的有效性和可靠性的准确估计，以便这项技术的最终用户能够充分评估检测的成本效益。为了解决这些问题的牛亲子鉴定，亲子排除概率估计从品种面板数据，并预测的DNA测试的效力，在39个多父系交配组，涉及5960小牛和505公牛。这些交配组的亲子鉴定表明，大多数涉及未知公畜的可变比例，这影响了公畜分配的可靠性。基于二项或β-二项概率分布的数学模型用于估计来自涉及未知公畜的多公畜交配的单公畜分配的可靠性。当排除概率为0.99或更大时，在允许高达20%的未知公畜后，可实现98-99%的可靠性。当排除概率下降到0.90以下，并且有20%的未知公畜时，可靠性很差，从而使测试的益处受到质疑。这突出了DNA检测实验室提供排除能力至少为99%的亲子鉴定的必要性。
It is important for bovine DNA testing laboratories to provide the cattle industry with accurate estimates of the efficacy and reliability of DNA tests offered so that end users of this technology can adequately assess the cost-benefits of testing. To address these issues for bovine paternity testing, paternity exclusion probability estimates were obtained from breed panel data and were predictive of the efficacy of the DNA tests used in 39 multiple-sire mating groups, involving 5960 calves and 505 bulls. Paternity testing of these mating groups has demonstrated that the majority involve a variable proportion of unknown sires and this impacts on the reliability of sire allocation. Mathematical models based on binomial or beta-binomial probability distributions were used to estimate the reliability of single-sire allocations from multiple-sire matings involving unknown sires. Reliability of 98-99% is achieved when the exclusion probability is 0.99 or greater, after allowing for up to 20% unknown sires. When the exclusion probability drops below 0.90 and there are 20% unknown sires, the reliability is poor, bringing into question the benefits of testing. This highlights the need for DNA testing laboratories to offer paternity tests with an exclusion power of at least 99%.