[In vitro and in vivo recoveries of cutaneous micro-dialysis probe of paeonol, eugenol and piperine].
[In vitro and in vivo recoveries of cutaneous micro-dialysis probe of paeonol, eugenol and piperine].
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DOI：
10.4268/cjcmm20162226
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
C. Yang;Jie Bai;S. Du;Yahua Cui;Qin-Shuai Zhang;Junming Ma
中科院分区：
文献类型：
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作者：
C. Yang;Jie Bai;S. Du;Yahua Cui;Qin-Shuai Zhang;Junming Ma
建立活血止痛贴中丹皮酚、丁香酚、胡椒碱微透析回收率检测方法，为进一步的经皮药动学研究提供依据。通过HPLC测定透析液中丹皮酚、丁子香酚和胡椒碱的浓度，并分别计算探针递送量。考察了浓度和校准方式对三种组分微透析探针输送量的影响，并研究了它们的探针吸收性、体内外稳定性和重复性。结果表明，以30%酒精为灌流液时，探针中丹皮酚、丁子香酚和胡椒碱含量很少，并且可以在短时间内从探针上清除。 3个组分的体内外探针递送量在8h内稳定，含药溶液和空白灌流液交替使用3次，3个组分的体内外探针递送量基本没有变化。不同浓度范围内丹皮酚、丁香酚、胡椒碱的体外回收率分别为（45.7±4.66）%、（27.82±2.95）%、（41.3±3.96）%，不存在浓度依赖性。在相同条件下，通过透析、逆透析和无净通量观察到相似的递送。因此，收集的级分的分析浓度可以通过体外或体内回收率来校准。同时，这也证明本研究建立的微透析方法适用于活血止痛贴的经皮药动学研究。
To establish a method for detecting micro-dialysis recovery of paeonol, eugenol and piperine in Huoxue Zhitong patch, in order to provide the basis for further percutaneous pharmacokinetics studies. The concentrations of paeonol, eugenol and piperine in dialysates were determined by HPLC, and probe deliveries were calculated respectively. The effects of concentration and calibration approaches on the micro-dialysis probe deliveries of the three components were investigated, and their probe absorbability, in vitro and in vivo probe stability and repeatability were also studied.The results indicated that little paeonol, eugenol and piperine were observed in probes with 30% alcohol as the perfusate, and could be cleaned from probe in a short time. And the in vivo and in vitro probe deliveries of three components were stable within 8 h, drug-containing solution and blank perfusate were alternatively used for three times, and the in vivo and in vitro probe deliveries of three components were basically unchanged. The in vitro recoveries of paeonol, eugenol and piperine with a range of concentration were respectively (45.7±4.66)%, (27.82±2.95)%, (41.3±3.96)%, which indicated no concentration independent. Under the same conditions, the similar delivery was observed by dialysis, retrodialysis and no-net flux. Therefore, the concentrations of analyses of the collected fraction could be calibrated by in vitro or in vivo recoveries. Meanwhile, this also proved that the micro-dialysis method built by this study is applicable to the study on percutaneous pharmacokinetics of Huoxue Zhitong patch.