GANGLIOSIDES ENHANCE NEURITE OUTGROWTH IN PC12 CELLS
GANGLIOSIDES ENHANCE NEURITE OUTGROWTH IN PC12 CELLS
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DOI：
10.1016/0165-3806(83)90006-8
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
GORIO, A
中科院分区：
文献类型：
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作者：
FERRARI, G;FABRIS, M;GORIO, A
神经节苷脂是一种表面膜成分，在神经元分化、生长和再生等细胞生理过程中发挥重要作用。[Rat选择[嗜铬细胞瘤] PC 12细胞作为模型系统来研究外源添加的糖磷脂对体外分化和再生的影响。牛脑神经节苷脂的混合物（GM 1 21%，GD 1a 39.7%，GD 1b 16%和GT 19%）或纯化的GM 1和GT分别在接种日加入到含有NGF的培养基中，并从第5天开始隔日监测它们的作用。纤维生长的程度和速度显着增强的培养基中含有神经节苷脂的浓度为10-6，10-7 M时，血清离开和10-3，10-4 M时，血清添加到培养基中。神经节苷脂的刺激作用需要神经生长因子的存在，以诱导神经突生长。然而，结合研究表明，外源性神经节苷脂不影响NGF [神经生长因子]与PC 12细胞的结合，因此它们的刺激作用可能与NGF及其受体之间的相互作用分开。经神经生长因子处理的细胞传代培养10天表明，神经节苷脂处理也增强了神经生长因子刺激的神经突起再生。神经节苷脂可以在细胞表面水平掺入，从而影响和促进神经突生长所涉及的膜现象。
Gangliosides are surface membrane components that have been suggested to play a significant role in the regulation of many cellular events including neuronal differentiation, growth and regeneration. [Rat pheochromocytoma] PC12 cells were chosen as a model system to study the influence of exogenously added glycosphyngolipids on in vitro differentiation and regeneration. A mixture of bovine brain gangliosides (GM1 21%, GD1a 39.7%, GD1b 16% and GT 19%) or purified GM1 and GT, respectively, were added to culture media containing NGF on plating day and their effect was monitored on alternate days starting on day 5. The degree and rate of fiber growth was significantly enhanced by media containing gangliosides at a concentration of 10-6, 10-7 M when serum was left out and 10-3, 10-4 M when serum was added to the culture medium. The stimulating ganglioside action required the presence of NGF to induce neurite outgrowth. However, binding studies indicated that exogenous gangliosides do not affect NGF [nerve growth factor] binding to PC12 cells, therefore their stimulatory action may be separated from the interaction between NGF and its receptors. Subculturing of NGF-treated cells for 10 days demonstrated that gangliosides treatment also enhanced the NGF stimulated regeneration of neurites. Gangliosides may be incorporated at the level of cell surface, thereby affecting and facilitating membrane phenomena involved in neurite outgrowth.