Total pregnancy-associated plasma protein A - a first trimester maternal serum marker for Down's syndrome: clinical and technical assessment of a poly-monoclonal enzyme immunoassay
Total pregnancy-associated plasma protein A - a first trimester maternal serum marker for Down's syndrome: clinical and technical assessment of a poly-monoclonal enzyme immunoassay
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DOI：
10.1080/00365510310002248
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发表时间：
2003-10-01
影响因子：
2.1
通讯作者：
Jaliashvili, I
中科院分区：
文献类型：
--
作者：
Christiansen, M;Jaliashvili, I
妊娠相关血浆蛋白A（PAPP-A）是胎儿染色体疾病的母体血清标志物，也是不良结局的风险标志物。PAPP-A在循环中以与嗜酸性粒细胞主要碱性蛋白（proMBP）的前体的2：2复合物（PAPP-A/proMBP）和二聚体PAPP-A存在。不含PAPP-A的proMBP复合物构成母体血清中proMBP的大部分。我们使用多克隆兔抗PAPP-A/proMBP抗体（SSI 6823）和单克隆鼠抗PAPP-A/proMBP抗体（HYB 234-3）开发并表征了PAPP-A的夹心酶免疫测定，所述抗体与PAPP-A/proMBP的PAPP-A部分反应。试验范围为2 mIU/L-500 mIU/L，试验内和试验间变异系数
Pregnancy-associated plasma protein A (PAPP-A) is a maternal serum marker of fetal chromosomal disease and a risk marker for adverse outcome. PAPP-A in the circulation exists both as a 2: 2 complex (PAPP-A/proMBP) with the proform of eosinophil major basic protein (proMBP) and as dimeric PAPP-A. Non-PAPP-A containing proMBP complexes constitute the bulk of proMBP in maternal serum. We developed and characterized a sandwich enzyme immunoassay for PAPP-A using a polyclonal rabbit anti-PAPP-A/proMBP antibody (SSI 6823) and a monoclonal murine anti-PAPP-A/proMBP antibody (HYB 234-3), reactive with the PAPP-A part of PAPP-A/proMBP. The assay range was 2 mIU/L-500 mIU/L, intra- and inter-assay coefficients of variation