Patterns of fos activation in rat raphe nuclei during feeding behavior
Patterns of fos activation in rat raphe nuclei during feeding behavior
复制标题
DOI：
10.1016/j.brainres.2008.01.036
复制
发表时间：
2008-03-20
期刊：
影响因子：
2.9
通讯作者：
Nogueira, Maria Ines
中科院分区：
文献类型：
--
作者：
Takase, Luiz Fernando;Nogueira, Maria Ines
为了分析中缝核在摄食行为不同阶段的补充差异，我们对大鼠进行了食物限制计划（每天进食2小时，共15天）。将动物置于不同的饲养条件下，分为寻找食物组（MFS）、摄食组（MFI）、饱腹感组（MFSa）和食物限制控制组（MFC）。基线条件组（BC）作为进一步的对照。MFI和MFC组表现出更大的自主神经和躯体激活，有更多的FOS-IR神经元。MFI组线性核（LRN）和背核（DRN）标记细胞较多；MFC组在正中核（MRN）、桥核（PRN）、大核（NRM）和隐核（NRO）中标记较多；MFSa组苍白球（NRP）中标记细胞较多。BC表现出最低的活性细胞数。PRN在中缝的激活率最高，DRN的激活率最低。进一步的实验显示，MFI组中缝核内几乎没有双标记（FOS-IR+ 5-HT-IR）细胞，这表明在食物摄入过程中中缝几乎没有血清素能激活。这些发现表明中缝核在摄食行为的不同阶段有不同的补充。这些发现可能反映了行为状态的变化（例如，食物引起的觉醒与睡眠），导致更大的运动激活，从而增加FOS表达。虽然这些数据与中缝系统作为自主和躯体活动的增益设置器的观点一致，但中缝的功能复杂性尚未完全了解。(c) 2008 Elsevier B.V.版权所有
To analyze the differential recruitment of the raphe nuclei during different phases of feeding behavior, rats were subjected to a food restriction schedule (food for 2 h/day, during 15 days). The animals were submitted to different feeding conditions, constituting the experimental groups: search for food (MFS), food ingestion (MFI), satiety (MFSa) and food restriction control (MFC). A baseline condition (BC) group was included as further control. The MFI and MFC groups, which presented greater autonomic and somatic activation, had more FOS-immunoreactive (FOS-IR) neurons. The MFI group presented more labeled cells in the linear (LRN) and dorsal (DRN) nuclei; the MFC group showed more labeling in the median (MRN), pontine (PRN), magnus (NRM) and obscurus (NRO) nuclei; and the MFSa group had more labeled cells in the pallidus (NRP). The BC exhibited the lowest number of reactive cells. The PRN presented the highest percentage of activation in the raphe while the DRN the lowest. Additional experiments revealed few double-labeled (FOS-IR+ 5-HT-IR) cells within the raphe nuclei in the MFI group, suggesting little serotonergic activation in the raphe during food ingestion. These findings suggest a differential recruitment of raphe nuclei during various phases of feeding behavior. Such findings may reflect changes in behavioral state (e.g., food-induced arousal versus sleep) that lead to greater motor activation, and consequently increased FOS expression. While these data are consistent with the idea that the raphe system acts as gain setter for autonomic and somatic activities, the functional complexity of the raphe is not completely understood. (c) 2008 Elsevier B.V. All rights reserved.