Impulse activity differentially regulates [Leu]enkephalin and catecholamine characters in the adrenal medulla.
Impulse activity differentially regulates [Leu]enkephalin and catecholamine characters in the adrenal medulla.
复制标题
脉冲活性差异性地调节肾上腺髓质中的亮氨酸脑啡肽和儿茶酚胺特征。
DOI：
10.1126/science.6144183
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Black,IB
中科院分区：
文献类型：
--
作者：
LaGamma,EF;Adler,JE;Black,IB
在体内和体外大鼠肾上腺髓质中检查了推定的肽神经体液[Leu]脑啡肽和儿茶酚胺能酶酪氨酸羟化酶和苯乙醇胺-N-甲基转移酶的调节。肾上腺去神经手术或突触传递的药物阻断，已知可降低儿茶酚胺特性的治疗，增加[Leu]脑啡肽含量。培养的延髓在4天内[Leu]脑啡肽增加了50倍，而酪氨酸羟化酶保持不变，苯乙醇胺-N-甲基转移酶下降到一个新的基线水平。藜芦碱诱导的去极化阻止了[Leu]脑啡肽的积累，这种作用被河豚毒素阻断，河豚毒素拮抗跨膜Na+内流。这些研究表明，脑啡肽和儿茶酚胺的字符是差异调节的冲动活动和去极化肾上腺髓质。
Regulation of the putative peptide neurohumour [Leu]enkephalin and the catecholaminergic enzymes tyrosine hydroxylase and phenylethanolamine-N-methyl-transferase was examined in the rat adrenal medulla in vivo and in vitro. Surgical denervation of the adrenal gland or pharmacologic blockade of synaptic transmission, treatments known to decrease catecholamine traits, increased [Leu]enkephalin content. Medullas explanted to culture exhibited a 50-fold rise in [Leu]enkephalin in 4 days, whereas tyrosine hydroxylase remained constant, and phenylethanolamine-N-methyltransferase decreased to a new baseline level. Veratridine-induced depolarization prevented the accumulation of [Leu]enkephalin, an effect that was blocked by tetrodotoxin, which antagonizes transmembrane Na+influx. These studies suggest that enkephalinergic and catecholamine characters are differentially regulated by impulse activity and depolarization in the adrenal medulla.