Mucosal injury and inflammation in a model of chronic granulomatous colitis in rats.
Mucosal injury and inflammation in a model of chronic granulomatous colitis in rats.
复制标题
大鼠慢性肉芽肿性结肠炎模型中的粘膜损伤和炎症。
DOI：
10.1016/0016-5085(93)91011-6
复制
发表时间：
1993
期刊：
影响因子：
29.4
通讯作者：
Grisham,MB
中科院分区：
文献类型：
--
作者：
Yamada,T;Sartor,RB;Marshall,S;Specian,RD;Grisham,MB
Background:The objective of this study was to characterize the acute and chronic inflammation induced by the intramural injection of peptidoglycan-polysaccharide (PG-PS) into the distal colon of genetically susceptible rats.Methods:Blood-to-lumen clearance of51Cr-ethylenediaminetetraacetic acid, colonic myeloperoxidase activity, colon weight, and plasma nitrite and nitrate levels were determined to quantitate colonic mucosal injury, inflammation, and nitric oxide (NO) production, respectively.Results:Intramural injection of PG-PS into the distal colon produced a local biphasic inflammatory response composed of an acute episode 3 days after injection; this was followed by a spontaneous reactivation of chronic granulomatous colitis manifested by colonic thickening, adhesions, and infiltration of the submucosa and muscularis propria with macrophages, neutrophils, and lymphocytes at 3–4 weeks. Mucosal ulcers were evident only at 3 weeks, but hepatic nodules, splenic necrosis, and arthritis were evident at both 3 and 4 weeks after PG-PS injection. PG-PS produced significant increases in colonic mucosal permeability, myeloperoxidase activity, and plasma nitrite and nitrate levels at 3 weeks postinjection compared with controls. PG-PS stimulated the production of nitrite by elicited peritoneal macrophages and neutrophils in vitro.Conclusions:PG-PS produces a chronic granulomatous colitis in rats; this colitis is characterized by enhanced NO production.
