Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.
Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.
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分离和鉴定对应于HLA-DR抗原β链的cDNA克隆。
DOI：
10.1073/pnas.79.6.1703
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发表时间：
1982
影响因子：
11.1
通讯作者：
L. Rask
中科院分区：
文献类型：
--
作者：
K. Wiman;D. Larhammar;L. Claesson;K. Gustafsson;L. Schenning;P. Bill;J. Böhme;M. Denaro;B. Dobberstein;U. Hammerling;S. Kvist;B. Servenius;J. Sundelin;P. A. Peterson;L. Rask
主要组织相容性复合体中的HLA-D基因座控制遗传多态性HLA-DR抗原的表达。从人淋巴母细胞样细胞系Raji部分纯化编码这些抗原的β链的mRNA。将mRNA复制成双链cDNA并克隆到大肠杆菌中。通过杂交选择获得的一个克隆pDR-β-1携带1070个碱基对的插入物，该插入物包括除信号序列之外的所有编码区和非翻译区的大部分。为了鉴定pDR-β-1，对来源于Raji细胞的高度纯化的HLA-DR抗原β链进行NH 2-末端氨基酸序列测定。该序列与从pDR-β-1编码区5 ′端的核苷酸序列推断的序列具有广泛的同源性。总之，氨基酸和核苷酸序列强烈支持Raji细胞含有至少两个β链基因座。
The HLA-D locus in the major histocompatibility complex controls the expression of the genetically polymorphic HLA-DR antigens. mRNA coding for the beta chains of these antigens was partially purified from the human lymphoblastoid cell line Raji. The mRNA was copied into double-stranded cDNA and cloned in Escherichia coli. One clone, pDR-beta-1, obtained by hybrid selection, carries a 1070-base-pair insert comprising all of the coding region except the signal sequence and a substantial portion of the untranslated region. To identify pDR-beta-1, highly purified HLA-DR antigen beta chains derived from Raji cells were subjected to NH2-terminal amino acid sequence determination. This sequence displayed extensive homology with that deduced from the nucleotide sequence at the 5' end of the pDR-beta-1 coding region. Taken together, the amino acid and nucleotide sequences strongly argue in favor of Raji cells containing at least two beta-chain loci.