RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi
RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi
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RpoE 通过调节伤寒沙门氏菌中的 SPI-1 和 SPI-2 促进侵袭和细胞内存活。
DOI：
10.2217/fmb.16.19
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发表时间：
2016-08-01
影响因子：
3.1
通讯作者：
Du, Hong
中科院分区：
文献类型：
--
作者：
Zhang, Haifang;Jia, Yanwei;Du, Hong
目的：探讨RpoE在沙门氏菌高渗应激后期的作用。材料与方法：应用基因芯片技术检测高渗应激120min后SPI-1和SPI-2的表达，并比较野生型和Delta rpoE株的侵袭能力和细胞内存活情况。在高渗胁迫120分钟后，检测了野生型和Delta rpoE菌株之间细菌蛋白的全球差异表达。结果：在Delta rpoE株中，SPI-1和SPI-2受到抑制，侵袭力和细胞内存活能力下降。13个细菌相关蛋白和11个分泌蛋白在野生型和Delta rpoE株之间存在显著差异。结论：RpoE可能通过调节SPI-1和SPI-2的表达促进肿瘤的侵袭和细胞内存活。
Aim: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella. Materials & methods: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and Delta rpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and Delta rpoE strains was examined after 120 min of hyperosmotic stress. Results: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the Delta rpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and Delta rpoE strains. Conclusion: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.