Influence of sublethal concentrations of antibiotics on the expression of the mannose-specific ligand of Escherichia coli
Influence of sublethal concentrations of antibiotics on the expression of the mannose-specific ligand of Escherichia coli
复制标题
抗生素亚致死浓度对大肠杆菌甘露糖特异性配体表达的影响
DOI：
10.1128/iai.28.1.154-159.1980
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
I. Ofek
中科院分区：
文献类型：
--
作者：
B. Eisenstein;E. Beachey;I. Ofek
在一些菌株中，亚抑制浓度的链霉素对大肠杆菌甘露糖结合活性的获得有不同的抑制能力，但没有一个核糖体突变的菌株对高水平的链霉素耐药，rpsL。我们还确定，细菌在其他抗生素中的生长，特别是那些干扰蛋白质合成的抗生素，导致甘露糖结合活性（通过酵母细胞凝集测量），亲和性程度（通过电子显微镜测量）降低，并与人类口腔上皮细胞粘附。氨基糖苷类抗生素链霉素、庆大霉素和新霉素的最低抑菌浓度影响最显著，其次是四环素。大观霉素和氯霉素对粘附性的影响都大于对粘附性的影响，尽管大观霉素对甘露糖结合活性的影响比氯霉素更明显。我们得出结论，抗生素浓度低于其最低抑制浓度时，可能对细菌的表面特性产生深远影响，这可能与细菌定植和感染人类粘膜表面的能力有关。其机制可能因药物而异，但似乎取决于抗生素抑制蛋白质合成的经典作用。
The ability of streptomycin, in subinhibitory concentrations, to differentially suppress the acquisition of the mannose-binding activity of Escherichia coli was demonstrated in several strains, but not one with a ribosomal mutation to high-level streptomycin resistance, rpsL. We also determined that the growth of bacteria in other antibiotics, notably those that interfere with protein synthesis, resulted in diminished mannose-binding activity (as measured by yeast cell agglutination), degree of piliation (as measured by electron microscopy), and adherence to human oral epithelial cells. The aminoglycoside antibiotics streptomycin, gentamicin, and neomycin had the most marked effects relative to their minimum inhibitory concentrations, followed by tetracycline. Both spectinomycin and chloramphenicol had more effect on adherence than on piliation, although spectinomycin had a more pronounced effect on mannose-binding activity than did chloramphenicol. We conclude that antibiotics, at concentrations below their minimum inhibitory concentration, may have profound effects on surface properties of bacteria that may be pertinent for their ability to colonize and infect human mucosal surfaces. The mechanism(s) may vary from one drug to another, but appear to depend on the classic actions of the antibiotics on inhibiting protein synthesis.