Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142
Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142
复制标题
普通小麦品种秦农142条锈病抗性位点的全基因组定位
DOI：
10.1094/pdis-05-18-0846-re
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发表时间：
2019
期刊：
影响因子：
4.5
通讯作者：
Chen X. M.
中科院分区：
文献类型：
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作者：
Zeng Qingdong;Wu Jianhui;Liu Shengjie;Chen Xianming;Yuan Fengping;Su Pingping;Wang Qilin;Huang Shuo;Mu Jingmei;Han Dejun;Kang Zhensheng;Chen X. M.
条锈病由条锈菌（Pucciniastriiformisf.）小麦黑胫病威胁着世界小麦生产。培育抗病品种是防治该病的最佳途径。中国小麦品种秦农142（QN142）对条锈病具有高水平的成株抗性。为了鉴定与抗条锈性相关的数量性状位点，以QN142和感病品种Avocet S为材料，构建了重组自交系（RIL）群体。在6个地点年环境的重复田间试验中，对亲本和165个F6重组自交系的条锈病感染类型和病害严重程度进行了评价。用单核苷酸多态性（SNP）标记对亲本和RIL进行基因分型。在QN142中检测到4个稳定的QTL，定位在染色体臂1BL、2AL、2BL和6BS上。1BL QTL可能是已知的抗病基因Yr 29，2BL QTL位于抗病基因富集区，2AL和6BS QTL可能是新的。竞争等位基因特异性聚合酶链反应标记开发的SNP标记侧翼这些QTL是高度多态性的150个小麦品种和育种系的面板。这些标记可用于小麦抗条锈QTL的分子标记辅助选择。
Stripe rust caused byPuccinia striiformisf. sp.triticithreatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance geneYr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.