Eradication of multidrug-resistant A. baumannii in burn wounds by antiseptic pulsed electric field
Eradication of multidrug-resistant A. baumannii in burn wounds by antiseptic pulsed electric field
复制标题
DOI：
10.1142/s2339547814500101
复制
发表时间：
2014-06-01
期刊：
影响因子：
--
通讯作者：
Yarmush, Martin L.
中科院分区：
文献类型：
--
作者：
Golberg, Alexander;Broelsch, G. Felix;Yarmush, Martin L.
新出现的细菌对多种药物的耐药性是烧伤创面管理中日益严重的问题。烧伤创面消毒需要新的非药物干预措施。在这里，我们报告了一种新的物理消毒方法：抗菌脉冲电场（PEF）应用于感染烧伤的外部。在小鼠模型中，我们发现PEF可以通过生物发光成像将全层烧伤创面中存在的多药耐药鲍曼不动杆菌的负荷降低4个数量级以上。此外，使用有限元数值模型，我们证明了PEF为烧伤创面提供非热、均匀、全层处理，从而克服了许多局部抗菌剂治疗深度的限制。这些建模工具和我们的体内结果将对PEF技术进一步转化为临床环境非常有用，因为它们为电极设计和治疗方案的规划提供了基本要素。
Emerging bacterial resistance to multiple drugs is an increasing problem in burn wound management. New non-pharmacologic interventions are needed for burn wound disinfection. Here we report on a novel physical method for disinfection: antiseptic pulsed electric field (PEF) applied externally to the infected burns. In a mice model, we show that PEF can reduce the load of multidrug resistant Acinetobacter baumannii present in a full thickness burn wound by more than four orders of magnitude, as detected by bioluminescence imaging. Furthermore, using a finite element numerical model, we demonstrate that PEF provides non-thermal, homogeneous, full thickness treatment for the burn wound, thus, overcoming the limitation of treatment depth for many topical antimicrobials. These modeling tools and our in vivo results will be extremely useful for further translation of the PEF technology to the clinical setting, as they provide the essential elements for planning of electrode design and treatment protocol.