Diversity of endophytic bacteria within nodules of the Sphaerophysa salsula in different regions of Loess Plateau in China
Diversity of endophytic bacteria within nodules of the Sphaerophysa salsula in different regions of Loess Plateau in China
复制标题
黄土高原不同地区萨尔苏拉球藻根瘤内生细菌多样性
DOI：
10.1111/j.1574-6941.2011.01063.x
复制
发表时间：
2011-06-01
影响因子：
4.2
通讯作者：
Wei, Ge Hong
中科院分区：
文献类型：
--
作者：
Deng, Zhen Shan;Zhao, Long Fei;Wei, Ge Hong
从中国黄土高原两个生态区野生豆科植物苦豆（Sphaerophysa salsula）根瘤中分离到115株内生细菌。通过限制性片段长度多态性分析、16S rRNA基因测序和肠杆菌重复基因间共有序列PCR（repeatingintegenicconsensus-PCR）分析，揭示了菌株的遗传多样性和遗传多样性。通过产孢试验和nifH基因序列分析，检测了它们的共生能力。这是首次系统地研究了S.盐草根瘤。发现的50个菌株是共生细菌，属于Mesorhizobium、Rhizobium和Sinorhizobium属的8个推定物种，具有相似的nifH基因;非共生菌65株，分属副球菌属、鞘氨醇单胞菌属、Inquilinus属、假单胞菌属、沙雷氏菌属、分枝杆菌属、诺卡氏菌属、链霉菌属、类芽孢杆菌属、短芽孢杆菌属、葡萄球菌属、根瘤中普遍存在与共生菌共存的赖氨酸芽孢杆菌和芽孢杆菌。与其他类似研究不同，本研究首次从同一根瘤中同时分离出共生和非共生细菌，为准确揭示这两种细菌之间的相关性提供了可能。这些结果为了解共生细菌、非共生细菌及其生境之间的相互作用提供了有价值的信息。
A total of 115 endophytic bacteria were isolated from root nodules of the wild legume Sphaerophysa salsula grown in two ecological regions of Loess Plateau in China. The genetic diversity and phylogeny of the strains were revealed by restriction fragment length polymorphism and sequencing of 16S rRNA gene and enterobacterial repetitive intergenic consensus-PCR. Their symbiotic capacity was checked by nodulation tests and analysis of nifH gene sequence. This is the first systematic study on endophytic bacteria associated with S. salsula root nodules. Fifty of the strains found were symbiotic bacteria belonging to eight putative species in the genera Mesorhizobium, Rhizobium and Sinorhizobium, harboring similar nifH genes; Mesorhizobium gobiense was the main group and 65 strains were nonsymbiotic bacteria related to 17 species in the genera Paracoccus, Sphingomonas, Inquilinus, Pseudomonas, Serratia, Mycobacterium, Nocardia, Streptomyces, Paenibacillus, Brevibacillus, Staphylococcus, Lysinibacillus and Bacillus, which were universally coexistent with symbiotic bacteria in the nodules. Differing from other similar studies, the present study is the first time that symbiotic and nonsymbiotic bacteria have been simultaneously isolated from the same root nodules, offering the possibility to accurately reveal the correlation between these two kinds of bacteria. These results provide valuable information about the interactions among the symbiotic bacteria, nonsymbiotic bacteria and their habitats.