Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation
Ultraviolet light-mediated photofunctionalization of titanium to promote human mesenchymal stem cell migration, attachment, proliferation and differentiation
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DOI：
10.1016/j.actbio.2009.04.022
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发表时间：
2009-10-01
影响因子：
9.7
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Aita, Hideki;Att, Wael;Ogawa, Takahiro
提高钛种植体的骨传导能力一直是牙科和矫形外科领域的研究热点。本研究测定了紫外光处理的钛的生物活性。人骨髓间充质干细胞(MSCs)经UVA(峰值波长为360 nm)或UVC(峰值波长为250 nm)处理48h后，在酸蚀微形貌钛表面进行培养。在培养的前3小时，迁移到UVC处理表面的细胞数量是迁移到未处理表面的细胞数量的8倍。孵育24小时后，经UVC处理的细胞数量是未处理表面的3倍以上。在经UVC处理的表面，粘着斑蛋白vinculin的广泛而密集的表达加速了细胞的扩散。经UVC处理的细胞表面溴脱氧尿嘧啶核苷掺入量增加三倍，碱性磷酸酶阳性面积增加一倍，骨相关基因表达上调，表明细胞增殖和分化加快。经UVC处理的表面对细胞的活力没有不利影响。这些生物效应在UVA治疗后没有看到，尽管产生了超亲水性。因此，我们发现了一种新的二氧化钛光功能化方法，它可以显著增强其在人MSCs中的生物活性。需要进一步的研究来研究这种表面修饰对不同化学成分和质地的含钛材料的普遍有效性，以及了解其在提高体内骨传导性方面的意义。(C)2009年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Improving the osteoconductive potential of titanium implants has been of continuing interest in the fields of dentistry and orthopedic surgery. This study determined the bioactivity of ultraviolet (UV) light-treated titanium. Human mesenchymal stem cells (MSCs) were cultured on acid-etched micro topographical titanium surfaces with and without 48 h pretreatment with UVA (peak wavelength of 360 nm) or UVC (peak wavelength of 250 nm). The number of cells that migrated to the UVC-treated surface during the first 3 h of incubation was eight times higher than those that migrated to the untreated surface. After 24 h of incubation, the number of cells attached to the UVC-treated surface was over three times more than those attached to the untreated surface. On the UVC-treated surface, the cellular spread was expedited with an extensive and intensive expression of the focal adhesion protein vinculin. The cells on the UVC-treated surface exhibited a threefold higher bromodeoxyuridine incorporation, a doubling of the alkaline phosphatase-positive area and the up-regulated expression of bone-related genes, indicating the accelerated proliferation and differentiation. The UVC-treated surface did not adversely affect the viability of the cells. These biological effects were not seen after UVA treatment, despite the generation of superhydrophilicity. Thus, we discovered a novel photofunctionalization of titanium dioxide that substantially enhances its bioactivity in human MSCs. Further studies are required to investigate the universal effectiveness of this surface modification for different titanium-containing materials, with varying chemistries and textures, as well as to understand its significance in enhancing in vivo osteoconductivity. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.