Hepatoprotective effects of ethanol extracts from Folium Syringae against acetaminophen-induced hepatotoxicity in vitro and in vivo
Hepatoprotective effects of ethanol extracts from Folium Syringae against acetaminophen-induced hepatotoxicity in vitro and in vivo
复制标题
丁香叶乙醇提取物对乙酰氨基酚引起的体内外肝毒性的保肝作用
DOI：
10.1016/j.jcma.2017.03.007
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发表时间：
2017
影响因子：
3
通讯作者：
Zhi Li
中科院分区：
文献类型：
--
作者：
Chen-Xi Shi;Yue-Xia Lin;Fang-Ping Liu;Yi-Cong Chang;Rui Li;Chang-Wen Li;Ying Li;Jing-Shan He;Xin Ma;Zhi Li
BackgroundThe leaves ofFolium Syringae(FS) have been long used as a traditional Chinese folk medicine for their anti-inflammatory effect, utilized as an antibacterial and antiviral treatment. The purpose of this study was to investigate the potential hepatoprotective effects of FS on acetaminophen-induced hepatic injury in primary hepatocytes and mice.MethodsHepatocytes obtained by the inverse perfusion method were divided randomly into five groups. Prior to acetaminophen exposure, 3 different doses of FS ethanol extracts were given to hepatocytes and mice, respectively. Thereafter, transaminases, glutathione S-transferase A1 (GSTA1) and some hepatic indices were determined.ResultsFS ethanol extracts (200 μg/mL) pretreatment prevented all of the alterations, returning their levels to nearly those levels observed in the control groupin vitro. Treatment with FS ethanol extracts (200 mg/kg) significantly reduced the toxicity induced by acetaminophenin vivo, which manifested as a decrease in transaminases, and the hepatoprotective effects of FS were similar to Silymarin (positive group). GSTA1 represented the same change trend as transaminases and hepatic indices, and at a dose of 100 μg/mL FS ethanol extractsin vitroand 100 mg/kgin vivo, GSTA1 content changed significantly (p< 0.01), but transaminases were insignificant (p> 0.05).ConclusionThe results of our investigation suggested that FS ethanol extracts possess significant protective effects against hepatotoxicity induced by acetaminophen bothin vitroandin vivo. In addition, GSTA1 could be used as an indicator assessing the extents of hepatic injury, which is more sensitive than transaminases.
