Advances in 2-phenylethanol production from engineered microorganisms
Advances in 2-phenylethanol production from engineered microorganisms
复制标题
利用工程微生物生产 2-苯乙醇的进展
DOI：
10.1016/j.biotechadv.2019.02.005
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发表时间：
2019
影响因子：
16
通讯作者：
Bin Zhuge
中科院分区：
文献类型：
--
作者：
Yuqin Wang;Hao Zhang;Xinyao Lu;Hong Zong;Bin Zhuge
2-苯乙醇（2-PE）是一种重要的香料成分，具有玫瑰般的气味。由于担心化学合成的2-PE中可能存在有毒副产物，消费者更喜欢天然芳香化合物，促进2-PE的生物合成。各种微生物天然产生2-PE，产量低。近年来，酵母和大肠杆菌的代谢工程策略在提高2-PE生物产量方面取得了巨大的成功，包括缓解反馈抑制、改善前体转运、提高关键酶的活性和减少副产物等。在这里，我们回顾了代谢工程策略应用于微生物增加生物生产的2-PE，解决目前的问题，并提出进一步的改进。
2-Phenylethanol (2-PE) is an important flavor ingredient with a rose-like odor. Due to concerns about the toxic byproducts potentially found in 2-PE from chemical synthesis, consumers prefer the natural aroma compound, promoting the biosynthesis of 2-PE. Various microorganisms produce 2-PE naturally with low yield. Recent metabolic engineering strategies in yeasts andEscherichia colihave achieved great success in improving 2-PE bioproduction, including the alleviation of feed-back inhibition, improvement of precursor transport, enhancing activities of crucial enzymes, and reduction of by-products. Here, we review the metabolic engineering strategies applied to microorganisms for increasing bioproduction of 2-PE, address current problems, and propose further improvements.