Screening for ergot alkaloid producers among microscopic fungi by means of the polymerase chain reaction
Screening for ergot alkaloid producers among microscopic fungi by means of the polymerase chain reaction
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DOI：
10.1023/a:1010403427611
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发表时间：
2001-05-01
期刊：
影响因子：
1.5
通讯作者：
Arinbasarov, MU
中科院分区：
文献类型：
--
作者：
Boichenko, LV;Boichenko, DM;Arinbasarov, MU
评价了聚合酶链反应检测青霉属和麦角菌属微生物中麦角生物碱生产者的潜力。使用了23株不同种类的真菌与先前研究的生物碱生产能力。利用简并引物扩增了麦角生物碱生物合成第一步催化酶4-二甲基烯丙基色氨酸合酶基因的内部片段。该方法揭示了一个约1.2kb的合成麦角生物碱的特异DNA片段，该片段具有完整的四环麦角林系统。产生具有修饰的C或D麦角林环的生物碱以及a-环匹阿尼酸的微生物没有产生预期大小的PCR片段。该片段在不能产生麦角生物碱的真菌中也没有发现。
The potential of the polymerase chain reaction for the detection of ergot alkaloid producers among microscopic fungi of the genera Penicillium and Claviceps was evaluated. Twenty-three strains of various species of fungi with a previously studied capacity for alkaloid production were used. The internal fragment of the gene encoding 4-dimethylallyltryptophan synthase, the enzyme catalyzing the first step in the biosynthesis of ergot alkaloids, was amplified using degenerate primers. This approach revealed an about 1.2-kb specific DNA fragment in micromycetes synthesizing ergot alkaloids with complete tetracyclic ergoline system. Microorganisms that produce alkaloids with modified C or D ergoline rings, as well as a-cyclopiazonic acid, did not yield the PCR fragment of the expected size. This fragment was also not found in fungi incapable of ergot alkaloid production.