Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle
Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle
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阳离子胆固醇修饰明胶作为体外 siRNA 递送载体
DOI：
10.1039/c2mb25424g
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
P. A. Kadengodlu
中科院分区：
文献类型：
--
作者：
R. Isaka;L. Yu;M. Sasaki;Y. Igarashi;H. Fuwa;P. A. Kadengodlu
测试通过阳离子胆固醇改性明胶的自组装制备的聚合物胶束用于siRNA递送。它在稳定表达荧光素酶基因的HeLa细胞中发挥了所需的基因敲除效果，并且与Lipofectamine® 2000相比，敲除能力提高了两倍。发现聚合物胶束表现出优异的稳定性，并增加了siRNA在血清中的生物稳定性。
Polymeric micelle prepared through the self-assembly of cationic cholesterol-modified gelatin was tested for siRNA delivery. It exerted the desired effect of gene knockdown in HeLa cells stably expressing the luciferase gene and achieved a two-fold increase in the knockdown ability when compared to Lipofectamine® 2000. It was found that the polymeric micelle exhibited excellent stability and increased the biological stability of the siRNA in serum.