In situ expression of interleukin-10 in noninflamed human gut and in inflammatory bowel disease
In situ expression of interleukin-10 in noninflamed human gut and in inflammatory bowel disease
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DOI：
10.1016/s0002-9440(10)65552-6
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发表时间：
1998-07-01
影响因子：
6
通讯作者：
Meuer, SC
中科院分区：
文献类型：
--
作者：
Autschbach, F;Braunstein, J;Meuer, SC
抗炎细胞因子如白细胞介素-10 （IL-10）的分泌失调可能在炎症性肠病（IBD）的发病机制中发挥作用。研究人员利用mRNA原位杂交和免疫组织化学方法研究了IL-10在克罗恩病（CD）、溃疡性结肠炎（UC）患者和对照组肠道组织中的表达。在研究的所有组织中，肠上皮细胞均表达IL-10 mRNA和IL-10蛋白，但在表达模式上无明显差异。然而，与非炎症肠道相比，炎症肠道中产生IL-10的单核细胞（MNCs）数量显著增加，无论是CD还是UC。这种细胞因子在巨噬细胞中富集的炎症浸润中最显著地表达，尽管T细胞似乎也有助于其产生。IBD中IL-10表达升高主要在粘膜下层，而IL-10在固有层细胞中的表达相对较低。相比之下，IL-1 β mRNA在固有层的表达优先增加。我们的数据反对IBD中IL-10产生的普遍缺乏。结果表明，IBD粘膜跨国公司局部产生的IL-10不足以下调固有层室中的促炎细胞因子如IL-1 β。
A dysregulated secretion of contra-inflammatory cytokines such as interleukin-10 (IL-10) could play a role in the pathogenesis of inflammatory bowel disease (IBD). Vile have investigated the expression of IL-10 in gut tissues from patients with Crohn's disease (CD), ulcerative colitis (UC) and controls by mRNA in situ hybridization and immunohistochemistry. Intestinal epithelial cells were found to express IL-10 mRNA and IL-10 protein in all of the tissues investigated without any major differences in the expression patterns. However, compared with noninflamed gut, significantly increased numbers of mononuclear cells (MNCs) producing IL-10 were present in inflamed gut, both in CD and UC. This cytokine was expressed most prominently by inflammatory infiltrates enriched in macrophages, although T cells seem to contribute to its production as well. Elevated IL-10 expression in IBD was mainly detected in the submucosa, whereas IL-10 production by lamina propria cells remained comparably low. In contrast, the expression of IL-1 beta mRNA was preferentially increased in the lamina propria. Our data argue against a general deficiency in IL-10 production in IBD. The results suggest rather that the local production of IL-10 by mucosal MNCs in IBD is insufficient to down-regulate pro-inflammatory cytokines such as IL-1 beta in the lamina propria compartment.