Endoglin promoter hypermethylation identifies a field defect in human primary esophageal cancer.
Endoglin promoter hypermethylation identifies a field defect in human primary esophageal cancer.
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内皮糖蛋白启动子高甲基化鉴定了人类原发性食管癌的场缺陷
DOI：
10.1002/cncr.28276
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发表时间：
2013-10-15
期刊：
影响因子：
6.2
通讯作者：
Meltzer, Stephen J.
中科院分区：
文献类型：
--
作者：
Jin, Zhe;Zhao, Zhenfu;Cheng, Yulan;Dong, Ming;Zhang, Xiaojing;Wang, Liang;Fan, Xinmin;Feng, Xianling;Mori, Yuriko;Meltzer, Stephen J.
内元（ENG）是一种180 kDa的跨膜糖蛋白，它是转化的生长因子-β受体复合物的成分，据报道，在几种人类癌症中，我们使用了2600个人类的Enderigation pristeration pristeration pristeration proferation，伊尔斯，显然是区别于食管鳞状细胞癌（ESCC）和食管腺癌（EAC）正常食管（n）（p <0.01），ESCC的归一化甲基化值显着高于N（p <0.01）或EAC频率为46.2％。肿瘤进展，至13.3％ Barrett的Metaplasia（BE），ESCC患者的N型ENC患者（平均值= 0.0186）的ENK EAC中的25％和26.9％的EAC EAC高度高。我们的Eng mRNA表达结论是，ENG的启动子过度甲基化是人ESCC中经常的组织特异性事件，并且在ESCC的早期检测中表现出具有承诺的生物标志物潜力的田间缺陷。
Endoglin (ENG) is a 180-kDa transmembrane glycoprotein that functions as a component of the transforming growth factor-β receptor complex. Recently, ENG promoter hypermethylation was reported in several human cancers. We examined ENG promoter hypermethylation using real-time quantitative methylation-specific PCR in 260 human esophageal tissues. ENG hypermethylation showed highly discriminative receiver-operator characteristic curve profiles, clearly distinguishing esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) from normal esophagus (N) (p<0.01). Interestingly, ENG normalized methylation values were significantly higher in ESCC than in N (p<0.01) or EAC (p<0.01). ENG hypermethylation frequency was 46.2% in ESCC and 11.9% in N, but increased early and sequentially during EAC-associated neoplastic progression, to 13.3% in Barrett’s metaplasia (BE), 25% in dysplastic BE (D), and 26.9% in frank EAC. ENG hypermethylation was significantly higher in N from ESCC patients (mean = 0.0186) than in N from EAC patients (mean = 0.0117; p < 0.05). Treatment of KYSE220 ESCC cells with the demethylating agent, 5-aza-2′-deoxycytidine, reversed ENG methylation and reactivated ENG mRNA expression. We conclude that promoter hypermethylation of ENG is a frequent, tissue-specific event in human ESCC and exhibits a field defect with promising biomarker potential for the early detection of ESCC. In addition, ENG hypermethylation occurs in a subset of human EAC, and early during BE-associated esophageal neoplastic progression.
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影响因子：
24.5
作者：
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影响因子：
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通讯作者：
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