Further evidence for supporting cell conversion in the damaged avian basilar papilla
Further evidence for supporting cell conversion in the damaged avian basilar papilla
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DOI：
10.1016/s0736-5748(96)00098-6
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发表时间：
1997-07-01
影响因子：
1.8
通讯作者：
Raphael, Y
中科院分区：
文献类型：
--
作者：
Adler, HJ;Komeda, M;Raphael, Y
两条证据表明，除了支持细胞分裂之外，还有一个过程可能会在噪音或耳毒性药物损伤的鸟类内耳中产生新的毛细胞。这个过程，支持细胞转换，发生在非分裂支持细胞转分化成毛细胞。首先，暴露于噪音的小鸡接受0，1或2天每天腹腔注射阿糖胞苷（DNA合成阻滞剂），以及每天两次腹腔注射溴脱氧尿苷，持续4天。处死后，对乳头进行溴脱氧尿苷免疫细胞化学处理。所有的耳朵都表现出分裂的细胞，但增加阿糖胞苷注射的数量减少了标记细胞的数量。事实上，每天两次阿糖胞苷注射几乎完全阻断了声音诱导损伤内短毛细胞区域的支持细胞分裂。这表明，尽管存在阿糖胞苷，但扫描电子显微镜在类似区域观察到的未配对的未成熟细胞可能是支持细胞转化的产物。在第二个实验中，用庆大霉素处理鸟类三天。在处理后6天处死时，处理乳头以进行光学和透射电子显微镜检查。观察到几种不寻常的细胞，具有毛细胞和支持细胞的表型特征。这些特殊的细胞可能处于从支持细胞表型向毛细胞表型的转变中。(C)1997年ISDN。
Two lines of evidence suggested that a process other than supporting cell divisions may give rise to new hair cells in the bird inner ear injured by either noise or ototoxic drugs. This process, supporting cell conversion, occurs when non-dividing supporting cells transdifferentiate into hair cells. First, noise-exposed chicks received zero, one or two daily i.p. injections of cytosine arabinoside (a DNA synthesis blocker), as well as two daily intraperitoneal injections of bromodeoxyuridine, for four days. Following sacrifice, the papillae were processed for bromodeoxyuridine immunocytochemistry. All the ears demonstrated dividing cells, but increasing the number of cytosine arabinoside injections decreased the number of labeled cells. Indeed, two cytosine arabinoside injections per day nearly completely blocked supporting cell divisions in the short hair cell region within the sound-induced lesion. This suggested that unpaired, immature cells observed in a similar region with scanning electron microscopy, despite the presence of cytosine arabinoside, may have been products of supporting cell conversion. In the second experiment, birds were treated with gentamicin for three days. Upon sacrifice at 6 days post-treatment, papillae were processed for light and transmission electron microscopy. Several unusual cells were observed with phenotypic features of both hair cells and supporting cells. The peculiar cells may be in a transition from the supporting cell phenotype to that of a hair cell. (C) 1997 ISDN.