Influence of neighbouring base sequence on N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis in the lacI gene of Escherichia coli.
Influence of neighbouring base sequence on N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis in the lacI gene of Escherichia coli.
复制标题
相邻碱基序列对大肠杆菌lacI基因N-甲基-N-硝基-N-亚硝基胍诱变的影响。
DOI：
10.1016/0022-2836(87)90668-1
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发表时间：
1987
影响因子：
5.6
通讯作者：
B. Glickman
中科院分区：
文献类型：
--
作者：
P. Burns;A. Gordon;B. Glickman
对N-甲基-N‘-硝基-N-亚硝基(MNNG)诱导的大肠埃希菌lacI基因前540个碱基对的正向突变进行了克隆和测序。总共有167个MNNG诱导的独立突变被表征，除了三个突变外，所有的突变都是从G·C到A·T的转变。这种突变特异性与鸟嘌呤O6位甲基化预测的错配是一致的。如此大量的突变的特征使得分析局部DNA序列对突变的影响成为可能。这一分析揭示了5‘侧翼基地的强烈影响。平均而言，在(5‘)之前有鸟嘌呤残基或腺嘌呤残基的鸟嘌呤残基在MNNG处理后发生突变的可能性分别是以嘧啶残基为首的那些鸟嘌呤残基的9倍和5倍。
N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced forward mutations within the first 540 base-pairs of thelacI gene ofEscherichia coliwere cloned and sequenced. In total, 167 MNNG-induced independent mutations were characterized, with G · C to A · T transitions accounting for all but three of the mutations. This mutagenic specificity is consistent with the mispairing predicted by the methylation of the O6position of guanine. The characterization of such large numbers of mutations permitted an analysis of the influence of local DNA sequence on mutagenesis. This analysis revealed a strong influence by the 5′ flanking base. On average, guanine residues preceded (5′) by a guanine or an adenine residue were, respectively, nine times and five times more likely to mutate after treatment with MNNG than those preceded by a pyrimidine residue.
O6-甲基鸟嘌呤突变和修复是不均匀的。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Topal,MD;Eadie,JS;Conrad,M
通讯作者：
Conrad,M