Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production.
Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production.
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DOI：
10.1038/srep08753
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发表时间：
2015-03-04
影响因子：
4.6
通讯作者：
Ito K
中科院分区：
文献类型：
--
作者：
Onda Y;Mochida K;Yoshida T;Sakurai T;Seymour RS;Umekawa Y;Pirintsos SA;Shinozaki K;Ito K
一些植物物种可以产生足够的热量，以提高其内部花的温度高于环境温度。在产热植物中，天南星在产热过程中表现出最高的呼吸活性。然而，与植物产热相关的基因的全面了解尚未实现。在这项研究中，我们进行了从头转录组分析的花器官，A。Concentrum。从头转录组组装总共代表了158，490个非冗余转录本，其中53，315个与已知基因具有显著同源性。为了探索与产热相关的基因，我们筛选了1266个转录本，这些转录本在每个样品的表达模式和温度趋势之间显示出显着的相关性。我们证实了五个假定的替代氧化酶转录本包括在过滤的转录本中。对过滤后转录本的基因本体论术语的富集分析表明，参与1-脱氧-d-木酮糖-5-磷酸合酶（DXS）活性的基因过度表达。DXS转录物在甲基-d-β 4-磷酸（MEP）途径中的表达谱与产热水平显著相关。我们的研究结果表明，MEP途径是产生香味单萜的主要生物合成途径。据我们所知，这是第一个报告描述的候选途径和关键酶的花香产生的产热植物。
Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-d-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-d-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants.