White collar-1, a central regulator of blue light responses in Neurospora, is a zinc finger protein
White collar-1, a central regulator of blue light responses in Neurospora, is a zinc finger protein
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DOI：
10.1002/j.1460-2075.1996.tb00510.x
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发表时间：
1996-04-01
期刊：
影响因子：
11.4
通讯作者：
Macino, G
中科院分区：
文献类型：
--
作者：
Ballario, P;Vittorioso, P;Macino, G
粗糙脉孢菌盲突变体白领 1 (wc-1) 在所有蓝光诱导现象中均存在多效性缺陷，从而确立了 wc-1 基因产物在信号转导途径中的作用。我们报道了通过染色体行走和突变体互补分离的 wc-1 基因的克隆。 wc-1 基因产物的阐明为蓝光信号转导难题提供了关键部分。 wc-1 基因编码 125 kDa 蛋白质，其编码基序包括单个四类锌指 DNA 结合域和富含谷氨酰胺的推定转录激活域。我们使用体外凝胶阻滞测定证明，在大肠杆菌中表达的 wc-1 锌指结构域能够特异性结合脉孢菌属蓝光调节基因的启动子。此外，我们发现 wc-1 基因表达是自动调节的，并由蓝光照射诱导转录。
The Neurospora crassa blind mutant white collar-1 (wc-1) is pleiotropically defective in all blue light-induced phenomena, establishing a role for the wc-1 gene product in the signal transduction pathway. We report the cloning of the wc-1 gene isolated by chromosome walking and mutant complementation. The elucidation of the wc-1 gene product provides a key piece of the blue light signal transduction puzzle. The wc-1 gene encodes a 125 kDa protein whose encoded motifs include a single class four, zinc finger DNA binding domain and a glutamine-rich putative transcription activation domain. We demonstrate that the wc-1 zinc finger domain, expressed in Escherichia coli, is able to bind specifically to the promoter of a blue light-regulated gene of Neurospora using an in vitro gel retardation assay. Furthermore, we show that wc-1 gene expression is autoregulated and is transcriptionally induced by blue light irradiation.