Rolling-circle amplification in DNA diagnostics: the power of simplicity.
Rolling-circle amplification in DNA diagnostics: the power of simplicity.
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DOI：
10.1586/14737159.2.6.542
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发表时间：
2002-11-01
影响因子：
5.1
通讯作者：
Demidov, Vadim V
中科院分区：
文献类型：
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作者：
Demidov, Vadim V
由于其稳健性和简单性，环状DNA探针的滚动复制在靶、探针或信号扩增的其他等温方法中在DNA诊断中占据独特的位置。综述了滚环扩增技术在DNA检测中的主要应用，重点介绍了滚环扩增技术的新概念和最新进展，并以单分子和单突变检测技术为例进行了介绍。基本机制，目前的争议和滚环放大的主要优点也被认为是。这种诊断方法的进一步发展的未来可能的方向进行了概述。
Due to its robustness and simplicity, the rolling replication of circular DNA probes holds a distinct position in DNA diagnostics among other isothermal methods of target, probe or signal amplification. Major rolling-circle amplification approaches to DNA detection via posthybridization probe/signal turn-by-turn enhancement are briefly overviewed here with an emphasis on the new concepts and latest progress in the field, including the single-molecule and single-mutation detection assays as exemplary applications. Underlying mechanisms, current controversies and principal advantages of rolling-circle amplification are also considered. Possible future directions for the further advancement of this diagnostic methodology are outlined.