Cyclooxygenase-2 in cancer cells and macrophages induces colon cancer cell growth by cigarette smoke extract
Cyclooxygenase-2 in cancer cells and macrophages induces colon cancer cell growth by cigarette smoke extract
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DOI：
10.1016/j.ejphar.2005.05.018
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发表时间：
2005-07-25
影响因子：
5
通讯作者：
Cho, CH
中科院分区：
文献类型：
--
作者：
Liu, ESL;Shin, VY;Cho, CH
吸烟、环氧化酶-2（考克斯-2）和巨噬细胞与结直肠癌独立相关。在本研究中，将香烟烟雾乙醇提取物应用于结肠癌细胞（SW 1，116）或间接通过活化的巨噬细胞（THP-1细胞），以证明其在体外和体内对癌细胞增殖和肿瘤生长的影响。乙醇提取物诱导SW 1116和THP-1细胞表达考克斯-2。THP-1预孵育液和乙醇提取物联合作用可进一步促进SW 1116细胞的增殖和考克斯-2表达，并促进肿瘤细胞增殖和血管生成，抑制肿瘤细胞凋亡。应用考克斯-2抑制剂（SC 236）减少肿瘤生长以及细胞增殖和血管生成。这些作用部分依赖于降低考克斯-2的表达。综上所述，抑制考克斯-2活性可能对预防吸烟者的结肠癌具有重要意义。(c)2005 Elsevier B.V保留所有权利。
Cigarette smoking, cycldoxygenase-2 (COX-2) and macrophages are independently associated with colorectal cancer. In the present study, cigarette smoke ethanol extract was applied to colon cancer cells (SW 1,116) or indirectly via activated macrophages (THP-1 cells) to attest their effects on cancer cell proliferation and tumor growth both in vitro and in vivo. Ethanol extract induced COX-2 expression in SW1116 and THP-1 cells. Combination of THP-1 pre-incubated medium and ethanol extract further potentiated COX-2 expression and proliferation of SW1116 cells.Tumor growth in nude mice was positively associated with the medium and/or ethanol extract treatments, together with the up-regulation of cell proliferation and angiogenesis, and down-regulation of apoptosis. Application of a COX-2 inhibitor (SC236) reduced tumor growth as well as cell proliferation and angiogenesis. These actions are partially depended on the decrease of COX-2 expression. Taken together, inhibition of COX-2 activity may have significant implication to prevent colon cancer in smokers. (c) 2005 Elsevier B.V All rights reserved.