A gold@silica core-shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection
A gold@silica core-shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection
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DOI：
10.1016/j.aca.2013.12.009
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发表时间：
2014-02-06
影响因子：
6.2
通讯作者：
Wu, Nianqiang
中科院分区：
文献类型：
--
作者：
Al-Ogaidi, Israa;Gou, Honglei;Wu, Nianqiang
与葡萄糖氧化酶（GOx）分子缀合的金纳米星@二氧化硅核壳纳米粒子已被开发为表面增强拉曼散射（SERS）生物传感器，用于无标记检测葡萄糖。表面固定的 GOx 酶催化葡萄糖氧化，产生过氧化氢。在激光激发下，Au nanostar@silica 纳米粒子附近产生的 H2O2 分子产生强烈的 SERS 信号，用于测量葡萄糖浓度。 Nanostar@silica 的 SERS 信号与基于 GOx 纳米颗粒的传感检测类似，显示出对水溶液中 25 μM 至 25 mM 葡萄糖浓度范围的动态响应，检测限为 16 μM。当葡萄糖与抗坏血酸和尿酸共存时，传感检测不会显示任何干扰。该传感器可应用于唾液样本。 (C) 2013 Elsevier B.V. 保留所有权利。
The gold nanostar@silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica similar to GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25 mu M to 25 mM in the aqueous solution with the limit of detection of 16 mu M. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample. (C) 2013 Elsevier B.V. All rights reserved.