Xenopus transgenics: methods using transposons.
Xenopus transgenics: methods using transposons.
复制标题
非洲爪蟾转基因：使用转座子的方法。
DOI：
10.1007/978-1-61779-992-1_14
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Mead,PaulE
中科院分区：
文献类型：
--
作者：
Kelley,ClairM;Yergeau,DonaldA;Zhu,Haiqing;Kuliyev,Emin;Mead,PaulE
转基因动物的产生是许多遗传策略的重要工具。DNA“剪切-粘贴”转座子系统可用于有效地修改xenopusgenome。位于环状质粒上的DNA转座子底物与编码同源转座酶的mRNA一起在单细胞期注射到受精的爪蟾胚胎中。细胞机制迅速翻译外源mRNA，产生活性转座子酶，催化转座子底物从质粒中切除并稳定整合到基因组DNA中。
The generation of transgenic animals is an essential tool for many genetic strategies. DNA “cut-and-paste” transposon systems can be used to efficiently modify theXenopusgenome. The DNA transposon substrate, harbored on a circularized plasmid, is co-injected into fertilizedXenopusembryos at the one-cell stage together with mRNA encoding the cognate transposase enzyme. The cellular machinery rapidly translates the exogenous mRNA to produce active transposase enzyme that catalyzes excision of the transposon substrate from the plasmid and stable integration into the genomic DNA.