THE FATE MAP OF THE CHICK FORELIMB-BUD AND ITS BEARING ON HYPOTHESIZED DEVELOPMENTAL CONTROL MECHANISMS
THE FATE MAP OF THE CHICK FORELIMB-BUD AND ITS BEARING ON HYPOTHESIZED DEVELOPMENTAL CONTROL MECHANISMS
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DOI：
10.1007/bf00326592
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
REEVE, AMF
中科院分区：
文献类型：
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作者：
BOWEN, J;HINCHLIFFE, JR;REEVE, AMF
碳颗粒和同位素鹌鹑移植物被用作标记，研究鸡前肢的命运图阶段20和27之间的显着特点。移植技术证实了碳方法的可靠性：它们都显示出惊人的不对称性，其中顶端中胚层组织在近端方向上逐渐移位（正如预期的基础上，通过净顶端添加组织的生长），但也在轴前方向上，而轴后组织在肢体向外生长的方向上变得细长。外胚层表现出类似的轴前-轴后不对称，但从最初的底层中胚层位移。与中胚层形成鲜明对比的是，远端外胚层与顶端外胚层脊保持恒定距离（或并入其中），从而意味着外胚层片在远端锚定并通过近端均匀拉伸而生长。在外胚层本身内，外周层相对于下面的表皮基底层向远端移位。外周中胚层的位移模式之间的外胚层和chordrogenic核心中胚层。有人认为，这样的形态发生现象是不可分割的组成部分的发展机制的意义，在控制模式生成。的解释和使用的命运地图的肢体发育的理论，特别是那些基于肢体轴定义的机制的影响，进行了审查。
Carbon particles and isotopic quail grafts were used as markers to study the salient features of the fate map of the chick forelimb between stages 20 and 27. The grafting technique confirmed the reliability of the carbon method: they both revealed striking asymmetries in which apical mesodermal tissue was progressively displaced in a proximal direction (as would be expected on the basis of growth by net apical addition of tissue) but also in a preaxial direction, while postaxial tissue became elongated in the direction of limb outgrowth. Ectoderm showed a similar preaxial-postaxial asymmetry but became displaced from initially underlying mesoderm. In marked contrast to mesoderm, distal ectoderm remained at a constant distance from the apical ectodermal ridge (or became incorporated into it), thus implying that the ectodermal sheet is anchored distally and grows by uniform stretching proximally. Within the ectoderm itself, the outer peridermal layer is displaced distally relative to the underlying epidermal basal layer. Peripheral mesoderm showed patterns of displacement which were intermediate between those of ectoderm and chrondrogenic core mesoderm. It is argued that such morphogenetic phenomena are integral components of developmental mechanisms of significance in the control of pattern generation. Implications of the interpretation and use of the fate map in relation to theories of limb development, particularly those based on mechanisms defined in terms of limb axes, are reviewed.