Insights into the phylogeny of the ciliate of class Colpodea based on multigene data.
Insights into the phylogeny of the ciliate of class Colpodea based on multigene data.
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DOI：
10.1002/ece3.9380
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发表时间：
2022-10
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
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在足亚纲中，分类群之间有许多尚未解决的进化关系。在这里，我们报告了包括SSU‐rRNA、ITS1‐5.8S‐ITS2 rRNA和线粒体小亚基核糖体RNA （mtSSU‐rRNA）基因在内的30个新序列，并基于每个基因和两个基因串联的数据集进行了系统发育分析。首次利用多基因分析了凉足亚纲的系统发育关系。主要发现有：(1)首次获得了C. reniformis和C. grandis的SSU‐rRNA、ITS1‐5.8S‐ITS2 rRNA和mtSSU‐rRNA基因序列，并将这两个物种归为C. inflata、C. lucida、C. cucullus和C. henneguyi；(2)聚类模式和形态相似性表明，盘状布氏蝇与蝎科亲缘关系较近；(3)将Emarginatophrya属的诊断改进为“Hausmanniellidae，最左侧纤毛列1 ~ 4列明显缩短且等长”，将Colpoda elliotti改为Emarginatophrya；(4)属肾形虫属仍非单元与添加10 5肾形虫属物种的种群序列,但基于目前只剩下两个肾形虫属组工作:集团我由c . inflata最亮的星,c .兜帽状,c . henneguyi c . reniformis和c .茅,第二组包括c . maupasi和c . ecaudata和斜槽和前庭打开可能的两个关键特性区分肾形虫属物种群体;(5)由于其分子关系密切，且与P. steinii具有高度相似的染色体运动模式、体细胞纤毛模式和口腔器官的基本组织，建议将其暂时划归到阴道科。在足亚纲中，分类群之间有许多尚未解决的进化关系。在这里，我们报告了30个新的序列，包括5个colpodeans的SSU‐rRNA， ITS1‐5.8S‐ITS2 rRNA和线粒体小亚基核糖体RNA （mtSSU‐rRNA）基因，并基于每个基因和两个基因连接的数据集进行了系统发育分析。首次利用多基因分析了凉足亚纲的系统发育关系。
In the class Colpodea, there are many unresolved evolutionary relationships among taxa. Here, we report 30 new sequences including SSU‐rRNA, ITS1‐5.8S‐ ITS2 rRNA, and the mitochondrial small subunit ribosomal RNA (mtSSU‐rRNA) genes of five colpodeans, and conduct phylogenetic analyses based on each individual gene and a two‐gene concatenated dataset. For the first time, multi‐genes were used to analyze phylogenetic relationships in the class Colpodea. The main findings are: (1) SSU‐rRNA, ITS1‐5.8S‐ ITS2 rRNA, and mtSSU‐rRNA gene sequences of C. reniformis and C. grandis are provided for the first time, and these two species group into the clade including C. inflata, C. lucida, C. cucullus, and C. henneguyi; (2) clustering pattern and morphological similarity indicate that Bresslauides discoideus has a close relation with Colpodidae spp.; (3) Emarginatophrya genus diagnosis is improved to be ‘Hausmanniellidae with sharply shortened and isometric leftmost 1‐4 ciliary rows’ and Colpoda elliotti is transferred to Emarginatophrya; (4) the genus Colpoda is still non‐monophyletic with the addition of 10 populations from five Colpoda species sequences, but there are only two Colpoda groups left based on the present work: Group I comprises C. inflata, C. lucida, C. cucullus, C. henneguyi, C. reniformis, and C. grandis, Group II comprises C. maupasi and C. ecaudata, and the presence of diagonal grooves and the way the vestibular opens might be the two key features that differentiates Colpoda species groups; (5) a close molecular relationship, and highly similar merotelokinetal mode, somatic ciliary pattern, and basic organization of the oral apparatus with P. steinii suggests Bromeliothrix metopoides should be temporarily assigned to Colpodidae. In the class Colpodea, there are many unresolved evolutionary relationships among taxa. Here, we report 30 new sequences, including SSU‐rRNA, ITS1‐5.8S‐ ITS2 rRNA, and the mitochondrial small subunit ribosomal RNA (mtSSU‐rRNA) genes of five colpodeans, and conduct phylogenetic analyses based on each individual gene and a two‐gene concatenated dataset. For the first time, multi‐genes were used to analyze phylogenetic relationships in the class Colpodea.
DOI：
10.1111/j.1550-7408.2009.00429.x
发表时间：
2009-09
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Foissner W;Stoeck T
通讯作者：
Stoeck T