Methanethiol-dependent dimethylsulfide production in soil environments.
Methanethiol-dependent dimethylsulfide production in soil environments.
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DOI：
10.1038/ismej.2017.105
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Todd JD
中科院分区：
文献类型：
--
作者：
Carrión O;Pratscher J;Curson ARJ;Williams BT;Rostant WG;Murrell JC;Todd JD
二甲基硫（DMS）是一种重要的环境痕量气体，在硫循环、向高等生物发出信号和大气化学中发挥作用。据信二甲基硫醚主要是在海洋环境中通过微生物降解渗透剂二甲基磺基丙酸酯（DMSP）而产生的。然而，陆地环境也会产生大量的DMS，例如，泥炭沼泽每天可以排放约6 μmol DMS m−2，可能是通过甲基乙烷（MeSH）的甲基化。一种名为“MddA”的甲基转移酶，在广泛的细菌和一些蓝藻中催化MeSH的甲基化，产生DMS，可能介导这一过程，因为mddA基因在陆地宏基因组中丰富。这是第一项研究调查MeSH依赖的DMS生产（MDD）在广泛的有氧环境中的功能。所有测试的土壤和海洋沉积物样品在与MeSH孵育时都会产生DMS。种植依赖和培养独立的方法被用来评估微生物群落的变化，除了在草原土壤中的MeSH，其中35.9%的细菌被预测含有mddA。在MeSH的存在下富集Methylotenera属的细菌。此外，还分离出许多新的Mdd+细菌菌株。尽管在草原土壤中的mddA的丰度，Mdd途径可能不是一个显着的DMS在这种环境中的来源，因为MeSH除了需要检测DMS在只有非常低的转换率。
Dimethylsulfide (DMS) is an environmentally important trace gas with roles in sulfur cycling, signalling to higher organisms and in atmospheric chemistry. DMS is believed to be predominantly produced in marine environments via microbial degradation of the osmolyte dimethylsulfoniopropionate (DMSP). However, significant amounts of DMS are also generated from terrestrial environments, for example, peat bogs can emit ~6 μmol DMS m−2 per day, likely via the methylation of methanethiol (MeSH). A methyltransferase enzyme termed ‘MddA’, which catalyses the methylation of MeSH, generating DMS, in a wide range of bacteria and some cyanobacteria, may mediate this process, as the mddA gene is abundant in terrestrial metagenomes. This is the first study investigating the functionality of MeSH-dependent DMS production (Mdd) in a wide range of aerobic environments. All soils and marine sediment samples tested produced DMS when incubated with MeSH. Cultivation-dependent and cultivation-independent methods were used to assess microbial community changes in response to MeSH addition in a grassland soil where 35.9% of the bacteria were predicted to contain mddA. Bacteria of the genus Methylotenera were enriched in the presence of MeSH. Furthermore, many novel Mdd+ bacterial strains were isolated. Despite the abundance of mddA in the grassland soil, the Mdd pathway may not be a significant source of DMS in this environment as MeSH addition was required to detect DMS at only very low conversion rates.
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DOI：
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发表时间：
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期刊：
The ISME journal
影响因子：
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作者：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
2002-01-01
影响因子：
3
作者：
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