Gated Trapped Ion Mobility Spectrometry Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
Gated Trapped Ion Mobility Spectrometry Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
复制标题
门控俘获离子淌度谱与傅里叶变换离子回旋共振质谱联用。
DOI：
10.1007/s12127-016-0197-0
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Park,MelvinA
中科院分区：
文献类型：
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作者：
Ridgeway,MarkE;Wolff,JeremyJ;Silveira,JoshuaA;Lin,Cheng;Costello,CatherineE;Park,MelvinA
离子迁移率谱联用质谱（IMS-MS）分析分子提供了分子的三维结构和质量的化学信息。由于两种设备在分析时间上存在很大差异，离子迁移率与俘获质谱仪的耦合一直具有挑战性。在本文中，我们提出了一种被称为“门控TIMS”的捕获离子迁移率（TIMS）分析方案的修改，该方案允许与傅里叶变换离子回旋共振（FT-ICR）分析仪有效耦合。对标准化合物进行了分析，并分析了源条件对不稳定泛素蛋白离子迁移谱产生的TIMS分布的影响。离子迁移分辨能力可达100。测量的泛素离子的碰撞截面与以前的测量结果在定性和定量上都非常一致。门控TIMS FT-ICR产生的结果可与使用TIMS/飞行时间MS仪器平台以及文献中发表的许多漂移管IMS-MS研究获得的结果相媲美。
Analysis of molecules by ion mobility spectrometry coupled with mass spectrometry (IMS-MS) provides chemical information on the three dimensional structure and mass of the molecules. The coupling of ion mobility to trapping mass spectrometers has historically been challenging due to the large differences in analysis time between the two devices. In this paper we present a modification of the trapped ion mobility (TIMS) analysis scheme termed “Gated TIMS” that allows efficient coupling to a Fourier Transform Ion Cyclotron Resonance (FT-ICR) analyzer. Analyses of standard compounds and the influence of source conditions on the TIMS distributions produced by ion mobility spectra of labile ubiquitin protein ions are presented. Ion mobility resolving powers up to 100 are observed. Measured collisional cross sections of ubiquitin ions are in excellent qualitative and quantitative agreement to previous measurements. Gated TIMS FT-ICR produces results comparable to those acquired using TIMS/time-of-flight MS instrument platforms as well as numerous drift tube IMS-MS studies published in the literature.