Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish.
Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish.
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DOI：
10.3390/ijms18061127
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发表时间：
2017-06-07
影响因子：
5.6
通讯作者：
Yin Z
中科院分区：
文献类型：
--
作者：
Dai X;Shu Y;Lou Q;Tian Q;Zhai G;Song J;Lu S;Yu H;He J;Yin Z
Piwi相互作用RNA（皮尔纳）生物发生的规律性对种系发育至关重要。作为Piwi相互作用蛋白，Tudor结构域相关蛋白（Tdrds）已被证明参与精子发生和皮尔纳途径。本研究对斑马鱼tdrd 12进行了鉴定，并观察了斑马鱼tdrd 12在母体细胞和生殖细胞中的表达模式。利用TALEN（转录激活因子样效应核酸酶）技术，产生两个独立的tdrd 12突变斑马鱼系。虽然没有发现缺陷的原始生殖细胞（PGCs）在tdrd 12-null鱼后代从杂合tdrd 12突变的父母，所有的Tdrd 12-缺陷的鱼发展成为不育的男性。受精后35天（dpf）生殖细胞的数量减少并最终丧失，导致Tdrd 12缺陷鱼的雄性化和不育。观察到tdrd 12突变体在性腺转换期生殖细胞减数分裂缺陷，揭示了Tdrd 12在配子发生中不可或缺的功能。我们的研究表明，斑马鱼Tdrd 12对生殖细胞的发育和维持是必不可少的。
The regularity of Piwi-interacting RNA (piRNA) biogenesis is crucial to germline development. Functioning as Piwi-interacting proteins, Tudor domain-related proteins (Tdrds) have been demonstrated to be involved in spermatogenesis and the piRNA pathway. In this study, zebrafish tdrd12 was identified, and the maternal and germ cell-specific expression patterns of zebrafish tdrd12 were observed. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent tdrd12 mutant zebrafish lines were generated. Although no defects were found during the generation of the primordial germ cells (PGCs) in the tdrd12-null fish progenies obtained from the heterozygous tdrd12 mutant parents, all Tdrd12-deficient fish developed into infertile males. The reduced numbers and eventually loss of the germ cells by 35 days post fertilization (dpf) led to masculinization and infertility of the Tdrd12-deficient fish. Meiosis defects of the germ cells in the tdrd12 mutants during the gonad-transitioning period were observed, revealing the indispensable functions of Tdrd12 in gametogenesis. Our studies demonstrated that zebrafish Tdrd12 is essential for germ cell development and maintenance.