Extension of the Lancefield classification for group A streptococci by addition of 22 new M protein gene sequence types from clinical isolates:: emm103 to emm124
Extension of the Lancefield classification for group A streptococci by addition of 22 new M protein gene sequence types from clinical isolates:: emm103 to emm124
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DOI：
10.1086/324621
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发表时间：
2002-01-01
影响因子：
11.8
通讯作者：
Beall, B
中科院分区：
文献类型：
--
作者：
Facklam, RF;Martin, DR;Beall, B
在过去的60年里，经典M蛋白血清分型在确定不同A群链球菌（GAS）菌株之间的关系以及这些细菌在世界范围内造成的各种临床表现方面具有不可估量的价值。尽管如此，在过去的20年里，继续扩展基于血清学的兰斯菲尔德气体分类方案的困难已经变得越来越明显。通过使用一种要求较低的基于序列的方法，该方法严格遵守先前建立的菌株标准，同时预测已知的M蛋白血清型，我们最近将emm94-emm102型添加到Lancefield方案中。现在建议通过将类型emm103添加到emm124继续扩展。与emm94-emm102型一样，这些新emm型中的每一种都有多个从严重疾病表现中恢复的独立分离株，每一种都是M蛋白不可分型的，国际GAS参考实验室可用的所有分型血清都无法分型，每一种都通过在正常人血液中增殖而在体外表现出抗吞噬特性。
Classic M protein serotyping has been invaluable during the past 60 years for the determination of relationships between different group A streptococci (GAS) strains and the varied clinical manifestations inflicted by these organisms worldwide. Nonetheless, during the past 20 years, the difficulties of continued expansion of the serology-based Lancefield classification scheme for GAS have become increasingly apparent. By use of a less demanding sequence-based methodology that closely adheres to previously established strain criteria while being predictive of known M protein serotypes, we recently added types emm94-emm102 to the Lancefield scheme. Continued expansion by the addition of types emm103 to emm124 are now proposed. As with types emm94-emm102, each of these new emm types was represented by multiple independent isolates recovered from serious disease manifestations, each was M protein nontypeable with all typing sera stocks available to international GAS reference laboratories, and each demonstrated antiphagocytic properties in vitro by multiplying in normal human blood.