NGF, BDNF, NT3, NT4 and GDNF in tooth development.
NGF, BDNF, NT3, NT4 and GDNF in tooth development.
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NGF、BDNF、NT3、NT4 和 GDNF 在牙齿发育中的作用。
DOI：
10.1111/j.1600-0722.1998.tb02158.x
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发表时间：
1998
影响因子：
1.9
通讯作者：
Olson,L
中科院分区：
文献类型：
--
作者：
Nosrat,CA;Fried,K;Ebendal,T;Olson,L
神经营养因子对神经元的发育、分化、维持和再生具有强大的作用。在本研究中，我们使用原位杂交技术来确定五种神经营养因子在牙齿发育过程中的基因活性位点。神经生长因子（NGF）和脑源性神经营养因子（BDNF）mRNA主要在出生后大鼠的牙乳头/牙髓中检测到，并且表达模式与牙神经支配的开始相关。与此相反，神经营养因子3（NT 3）和4（NT 4）mRNA的表达模式主要是上皮细胞和最强的早期发育阶段，牙齿还没有神经支配。胶质细胞源性神经营养因子（GDNF）mRNA在早期阶段存在于牙上皮中，但在发育后期，GDNF mRNA表达主要是间充质的，在成牙本质细胞层中观察到并延伸到成牙本质细胞下区。我们的研究结果表明，神经营养因子和GDNF可能在牙齿发育过程中具有多种功能。除了对牙齿神经支配的建立产生影响外，神经营养物质还可能具有形态发生作用，例如调节发育中的上皮细胞和间充质细胞的增殖或分化。
Neurotrophic factors have robust effects on development, differentiation, maintenance and regeneration of neurons. In the present study, we have usedin situhybridization to determine the specific sites of gene activity of five neurotrophic factors during tooth development. Nerve growth factor (NGF) and brain‐derived neurotrophic factor (BDNF) mRNAs were mainly detected in the dental papilla/pulp in postnatal rats, and the pattern of expression correlated with onset of dental innervation. In contrast, neurotrophin 3 (NT3) and 4 (NT4) mRNA expression patterns were predominantly epithelial and were strongest during early developmental stages when teeth are not yet innervated. Glial cell line‐derived neurotrophic factor (GDNF) mRNA was present in dental epithelium at early stages, but later in development, GDNF mRNA expression was mainly mesenchymal and observed in the odontoblast layer and extending into the subodontoblast zone. Our results suggest that both neurotrophins and GDNF may have multiple functions during tooth development. In addition to an influence on the establishment of the dental innervation, neurotrophic substances might have morphogenetic effects such as modulating the proliferation or differentiation of developing epithelial and mesenchymal cells.