Infectious transmission of human T‐cell leukemia virus to rabbits
Infectious transmission of human T‐cell leukemia virus to rabbits
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人类 T 细胞白血病病毒对兔子的传染性传播
DOI：
10.1002/ijc.2910350113
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发表时间：
1985
影响因子：
6.4
通讯作者：
M. Kobayashi
中科院分区：
文献类型：
--
作者：
I. Miyoshi;S. Yoshimoto;I. Kubonishi;M. Fujishita;Y. Ohtsuki;M. Yamashita;K. Yamato;S. Hirose;H. Taguchi;K. Niiya;M. Kobayashi
通过与携带人T细胞白血病病毒（HTLV）的人T细胞系（MT-2）共培养建立兔淋巴细胞系（Ra-I）。Ra-1细胞系是染色体雄性的，并且持续感染HTLV。将经或未经丝裂霉素C处理的Ra-1细胞静脉内接种（i. v.）的一个例子三只母兔所有3只动物均产生针对HTLV抗原的抗体。在存在T细胞生长因子（TCGF）的情况下培养这些血清转化者之一的淋巴细胞，在TCGF生长的染色体雌性淋巴细胞中检测到HTLV颗粒。将来自其他2例血清转化者的淋巴细胞与来自2例抗HTLV阴性健康男性的淋巴细胞共培养，建立了来自各个体的产HTLV T细胞系。将1只HTLV感染家兔的血液输注至2只雌性家兔中，也导致2只受体发生血清转化。从其中一例输血相关血清转换者中建立了携带HTLV的淋巴细胞系（Ra-2）。Ra-2细胞系最初是TCGF依赖性的，但后来变得不依赖于TCGF。这些结果表明，HTLV可以传播给兔子。这些动物为研究HTLV的传播方式和致病性提供了合适的模型系统。
A rabbit lymphoid cell line (Ra‐I) was established by co‐cultivation with a human T‐cell line (MT‐2) carrying human T‐cell leukemia virus (HTLV). The Ra‐l cell line is chromosomally male and is persistently infected with HTLV. Ra‐l cells, with or without mitomycin‐C treatment, were inoculated intravenously (i. v.) into 3 female rabbits. All 3 animals responded with the production of antibodies to HTLV antigens. Lymphocytes from one of these seroconverters were cultured in the presence of T‐cell growth factor (TCGF) and HTLV particles were detected in the TCGF‐grown lymphocytes which were chromosomally female. Co‐cultivation of lymphocytes from the 2 other seroconverters with lymphocytes from 2 anti‐HTLV‐negative healthy men gave rise to the establishment of an HTLV‐producing T‐cell line derived from each individual. Blood transfusion from one of the HTLV‐infected rabbits into 2 female rabbits also resulted in the seroconversion of both recipients. An HTLV‐carrying lymphoid cell line (Ra‐2) was established from one of the transfusion‐related seroconverters. The Ra‐2 cell line was initially TCGF‐dependent but later became TCGF‐independent. These results indicate that HTLV can be transmitted to rabbits. These animals may provide a suitable model system for studying the mode of transmission and pathogenicity of HTLV.