A regulatory role for Fcγ receptors CD16 and CD32 in the development of murine B cells
A regulatory role for Fcγ receptors CD16 and CD32 in the development of murine B cells
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Fcγ受体CD16和CD32在小鼠B细胞发育中的调节作用
DOI：
10.1182/blood.v92.8.2823.420k12_2823_2829
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发表时间：
1998
期刊：
影响因子：
20.3
通讯作者：
R. Lynch
中科院分区：
文献类型：
--
作者：
B. Andrés;A. Mueller;S. Verbeek;M. Sandor;R. Lynch
在发育早期，小鼠B系祖细胞表达两类IgG Fc受体（FcγR），命名为FcγRII（CD 32）和FcγRIII（CD 16），但成熟B淋巴细胞仅表达FcγRII（CD 32），当其被诱导与mIgM结合时，其作为B细胞活化的抑制剂发挥作用。CD 16和CD 32在B系前体细胞上的功能以前没有研究过。为了研究FcγR对发育中的B系细胞的功能，在存在2.4G2（一种结合CD 16和CD 32的大鼠单克隆抗体）或存在对照正常大鼠IgG的情况下培养正常小鼠骨髓细胞，然后分析B系隔室的作用。与对照培养物相比，含有2.4G2的培养物显示B系细胞的生长和分化增强。当来自正常骨髓的荧光激活细胞分选的B细胞前体（B220 +，sIgM-，HSA高，FcγR +）与骨髓基质细胞系BMS 2共培养时，也会出现2.4G2的增强作用，但当它们在BMS 2条件培养基中培养时则没有。由2.4G2诱导的B系发育的增强是CD 16依赖性和CD 32依赖性的，因为2.4G2不影响来自编码CD 16或CD 32的基因已被破坏的小鼠的骨髓培养物中的B系生长或分化。对来自CD 16基因破坏小鼠的新鲜骨髓的分析显示，B细胞隔室中的细胞数量和分布正常，但在CD 32基因破坏小鼠中，B细胞隔室显著扩大。这些实验提供了几条证据，表明鼠B细胞前体上表达的FcγR可影响其生长和分化。© 1998年美国血液学会。
Early in development, murine B-lineage progenitor cells express two classes of IgG Fc receptors (FcγR) designated as FcγRII (CD32) and FcγRIII (CD16), but mature B lymphocytes only express FcγRII (CD32), which functions as an inhibitor of B-cell activation when it is induced to associate with mIgM. The functions of CD16 and CD32 on B-lineage precursor cells have not previously been investigated. To search for FcγR functions on developing B-lineage cells, normal murine bone marrow cells were cultured in the presence of 2.4G2, a rat monoclonal antibody that binds to CD16 and CD32, or in the presence of control normal rat IgG, and then the B-lineage compartment was analyzed for effects. Cultures that contained 2.4G2 showed enhanced growth and differentiation of B-lineage cells compared with control cultures. The enhancing effect of 2.4G2 also occurred when fluorescence-activated cell-sorted B-cell precursors (B220 + , sIgM − , HSA high , FcγR + ) from normal bone marrow were cocultured with BMS2, a bone marrow stromal cell line, but not when they were cultured in BMS2-conditioned media. The enhancement of B-lineage development induced by 2.4G2 was CD16-dependent and CD32-dependent, because 2.4G2 did not effect B-lineage growth or differentiation in cultures of bone marrow from mice in which either the gene encoding CD16 or CD32 had been disrupted. Analysis of fresh bone marrow from the CD16 gene-disrupted mice showed normal numbers and distribution of cells within the B-cell compartment, but in CD32 gene-disrupted mice, the B-cell compartment was significantly enlarged. These experiments provide several lines of evidence that the FcγR expressed on murine B-cell precursors can influence their growth and differentiation. © 1998 by The American Society of Hematology.
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