What to do with “one‐hit wonders”?
What to do with “one‐hit wonders”?
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遇到“一击奇迹”怎么办？
作者：
T. Veenstra;T. Conrads;H. Issaq
全球蛋白质组学已经见证了基于溶液的革命，其中蛋白质的混合物被消化成肽，然后通过质谱（MS）进行分离和分析。与基于凝胶的方法形成鲜明对比的是，在MS分析之前，蛋白质混合物的复杂性增加而不是降低。这种“自下而上”的方法使得肽图谱作为蛋白质鉴定工具过时，因为典型的MS扫描将检测源自许多不同蛋白质的肽。相反，基于溶液的全球蛋白质组学研究依赖于串联MS来识别肽，然后将其外推为源自完整蛋白质。就使用基于溶液的方法鉴定的蛋白质数量而言，结果相当令人印象深刻，在此类研究中常规鉴定了1000多种蛋白质[1]。
Global proteomics has been witness to the solutionbased revolution in which mixtures of proteins are digested into peptides prior to fractionation and analysis by mass spectrometry (MS). In stark contrast to gelbased methods, the complexity of the protein mixture is increased rather than decreased prior to MS analysis. This “bottom up” approach makes peptide mapping as a protein identification tool obsolete as a typical MS scan will detect peptides originating from many different proteins. Instead, solution-based global proteomic studies rely on tandem MS to identify peptides, which are then extrapolated as originating from intact proteins. The results as far as the number of proteins identified using solution-based methods are quite impressive, with more than 1000 proteins being routinely identified in such studies [1].