Advanced In Vivo Heteronuclear MRS Approaches for Studying Brain Bioenergetics Driven by Mitochondria.
Advanced In Vivo Heteronuclear MRS Approaches for Studying Brain Bioenergetics Driven by Mitochondria.
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DOI：
10.1007/978-1-59745-543-5_15
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Chen W
中科院分区：
文献类型：
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作者：
Zhu XH;Du F;Zhang N;Zhang Y;Lei H;Zhang X;Qiao H;Ugurbil K;Chen W
活体磁共振波谱（MRS）方法用于生物医学研究的最大优点是它能够无创地测量活体器官内的各种代谢物。因此，它为确定人类和动物的代谢物、化学反应速率和生物能量学及其动态变化提供了宝贵的工具。由于检测灵敏度的显著提高和光谱分辨率的提高，体内MRS的能力在更高的磁场下进一步增强。体内磁共振成像技术的最新进展进一步证明了其在许多生物医学研究领域，特别是在脑研究方面的巨大潜力。在此，我们综述了体内异核31P和17O MRS方法的最新进展，以及它们在测定动物和人类大脑线粒体内氧和ATP代谢率方面的应用。
The greatest merit of in vivo magnetic resonance spectroscopy (MRS) methodology used in biomedical research is its ability for noninvasively measuring a variety of metabolites inside a living organ. It, therefore, provides an invaluable tool for determining metabolites, chemical reaction rates and bioenergetics, as well as their dynamic changes in the human and animal. The capability of in vivo MRS is further enhanced at higher magnetic fields because of significant gain in detection sensitivity and improvement in the spectral resolution. Recent progress of in vivo MRS technology has further demonstrated its great potential in many biomedical research areas, particularly in brain research. Here, we provide a review of new developments for in vivo heteronuclear 31P and 17O MRS approaches and their applications in determining the cerebral metabolic rates of oxygen and ATP inside the mitochondria, in both animal and human brains.