Challenges and clinical relevance of molecular detection of Bordetella pertussis in South Africa
Challenges and clinical relevance of molecular detection of Bordetella pertussis in South Africa
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DOI：
10.1186/s12879-019-3869-7
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发表时间：
2019-03-21
影响因子：
3.7
通讯作者：
von Gottberg, Anne
中科院分区：
文献类型：
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作者：
Moosa, Fahima;du Plessis, Mignon;von Gottberg, Anne
背景我们评估了多目标实时 PCR 检测方法在严重呼吸道疾病 (SRI)、流感样疾病 (ILI) 和无症状对照患者中检测和诊断百日咳博德特氏菌的效用。方法对鼻咽标本（SRI、ILI 和对照）和诱导痰 (SRI) 进行 IS481、pIS1001、hIS1001 和 ptxS1 的实时 PCR 检测2012年6月至2016年5月通过呼吸道疾病监测收集的数据。使用 PCR 循环阈值 (C-t) 值截止值，IS481 阳性病例被分类为确诊病例 (C-t
BackgroundWe assessed the utility of a multi-target, real-time PCR assay for Bordetella pertussis detection and diagnosis in patients with severe respiratory illness (SRI), influenza-like illness (ILI), and asymptomatic controls.MethodsReal-time PCR detection of IS481, pIS1001, hIS1001 and ptxS1 was performed on nasopharyngeal specimens (SRI, ILI and controls) and induced sputum (SRI) collected from June 2012 to May 2016 through respiratory illness surveillance. Using PCR cycle threshold (C-t) value cut-offs, IS481 positive cases were classified as confirmed (C-t