Different Timing of Changes in Mitochondrial Functions following Endurance Training
Different Timing of Changes in Mitochondrial Functions following Endurance Training
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DOI：
10.1249/mss.0b013e31822b0bd4
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发表时间：
2012-02-01
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影响因子：
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通讯作者：
Richard, Ruddy
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文献类型：
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作者：
Daussin, Frederic N.;Rasseneur, Laurence;Richard, Ruddy
道辛，F. N.，L.拉塞诺，J·博伊特比尔，A-L.查尔斯，S。P.杜福尔，B。杰尼Buffalle和R. Richard.耐力训练后线粒体功能变化的不同时间。医学科学体育锻炼：第44卷，第2期，第100页。217-224，2012。目的：本研究的目的是探讨耐力训练诱导的适应性的时间过程中的两个主要线粒体功能。研究方法：将40只大鼠分为4组：对照组和3个训练组：1-d训练组、5-d训练组和10-d训练组。训练方案包括在电动跑步机上跑步30分钟（26 m.min（-1），15%等级）。核呼吸因子-1;通过qPCR测量线粒体转录因子A;超氧化物歧化酶-2;谷胱甘肽过氧化物酶-4;和柠檬酸合酶（CS）信使RNA水平。线粒体呼吸和H2 O2释放进行了评估，使用渗透纤维的白色腓肠肌原位。在两种条件下进行自由基泄漏的计算，其中两种测量中的底物相同。CS活性通过电化学计量法评估。结果如下：早期的时间依赖性调制的信使RNA水平的观察与训练：核呼吸因子-1和超氧化物歧化酶-2水平增加急性运动后，转录因子A，线粒体和CS水平提高5天后，和谷胱甘肽过氧化物酶-4水平增加10天后。5天后CS活性提高了29% +/- 8%（P < 0.01），自由基泄漏减少了50% +/- 7%（P < 0.05）。10 d耐力训练对线粒体H2 O2释放无显著影响，但增加了线粒体呼吸速率（P < 0.05）。结论：我们的研究结果表明，线粒体适应遵循一个顺序的程序，其中线粒体呼吸和自由基泄漏的适应发生根据不同的时间。总的来说，这些结果表明早期线粒体质的适应耐力训练。
DAUSSIN, F. N., L. RASSENEUR, J. BOUITBIR, A-L. CHARLES, S. P. DUFOUR, B. GENY, Y. BURELLE, and R. RICHARD. Different Timing of Changes in Mitochondrial Functions following Endurance Training. Med. Sci. Sports Exerc., Vol. 44, No. 2, pp. 217-224, 2012. Purpose: The objective of this study was to investigate the time course of the endurance training-induced adaptations in two major mitochondrial functions. Methods: Forty rats were divided into four groups: a control group and three training groups-a 1-d training group, a 5-d training group, and a 10-d training group. The training protocol consisted of 30 min of running on a motorized treadmill (26 m.min(-1), 15% grade). Nuclear respiratory factor-1; transcription factor A, mitochondrial; superoxide dismutase-2; glutathione peroxidase-4; and citrate synthase (CS) messenger RNA levels were measured by qPCR. Mitochondrial respiration and H2O2 release were assessed using permeabilized fibers of white gastrocnemius in situ. Calculation of free radical leak was performed in two conditions where substrates were identical in both measurements. CS activity was assessed spectrophotometrically. Results: An early time-dependent modulation in messenger RNA levels was observed with training: nuclear respiratory factor-1 and superoxide dismutase-2 levels increased after acute exercise, transcription factor A, mitochondrial and CS levels improved after 5 d, and glutathione peroxidase-4 levels increased after 10 d. CS activity improved by 29% +/- 8% (P < 0.01) after 5 d together with a 50% +/- 7% reduction in the free radical leak (P < 0.05). Finally, 10 d of endurance training did not significantly alter mitochondrial H2O2 release but increased mitochondrial respiration rates in situ (P < 0.05). Conclusions: Our results demonstrate that mitochondrial adaptations follow a sequential program in which mitochondrial respiration and free radical leak adaptations occur according to a different timing. Collectively, these results suggest early mitochondrial qualitative adaptations in response to endurance training.