Cholinergic and α-Adrenergic Stimulation of Prostaglandin Release by Dog Thyroid in Vitro*
Cholinergic and α-Adrenergic Stimulation of Prostaglandin Release by Dog Thyroid in Vitro*
复制标题
犬甲状腺体外胆碱能和 α-肾上腺素能刺激前列腺素释放*
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
J. Dumont
中科院分区：
文献类型：
--
作者：
J. Boeynaems;M. Waelbroeck;J. Dumont
通过直接 RIA 测量，狗甲状腺切片在培养介质中释放前列腺素 E2 (PGE2)、F2 α (PGF2 α)、15-酮-13,14-二氢-F2 α 和血栓素 B2 (TxB2)。这种释放可以被吲哚美辛和萘普生抑制，表明它对应于新合成。氨甲酰胆碱（2--100微米）刺激PGE2、PGF2α和TxB2的释放，而肾上腺素（20微米至1nM）增强PGE2和PGF2α的释放，但对TxB2没有影响。这些刺激分别被阿托品和二氢麦角隐亭抑制，表明毒蕈碱受体和α-肾上腺素受体参与其中。离子载体 A23187 再现了这些刺激，并且在没有外源 Ca++ 的情况下以及 EGTA 耗尽后，氨甲酰胆碱和肾上腺素的刺激作用受到抑制。因此，Ca++ 似乎是甲状腺中 PG 产生的主要调节剂。 TSH (0.06--10 MU/ml)和二丁酰cAMP对体外狗甲状腺释放PGE2、PGF2α或TxB2没有影响。
Dog thyroid slices released in their incubation medium prostaglandins E2 (PGE2), F2 alpha (PGF2 alpha), 15-keto-13,14-dihydro-F2 alpha, and thromboxane B2 (TxB2), as measured by direct RIA. This release could be inhibited by indomethacin and naproxen, indicating that it corresponded to a neosynthesis. Carbamylcholine (2--100 microns) stimulated the release of PGE2, PGF2 alpha, and TxB2, whereas epinephrine (20 microns to 1 nM) enhanced the release of PGE2 and PGF2 alpha but had no effect on TxB2. These stimulations were inhibited by atropine and dihydroergocryptine, respectively, suggesting that a muscarinic and an alpha-adrenergic receptor were involved. The ionophore A23187 reproduced these stimulations, and the stimulatory effects of carbamylcholine and epinephrine were inhibited in the absence of exogenous Ca++ and after EGTA depletion. Ca++ thus appears as a major regulator of PG production in the thyroid. TSH (0.06--10 MU/ml) and dibutyryl cAMP had no effect on the release of PGE2, PGF2 alpha, or TxB2 by dog thyroid in vitro.