RNA encapsidation by SV40-derived nanoparticles follows a rapid two-state mechanism.
RNA encapsidation by SV40-derived nanoparticles follows a rapid two-state mechanism.
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DOI：
10.1021/ja2110703
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发表时间：
2012-05-30
影响因子：
15
通讯作者：
Raviv, Uri
中科院分区：
文献类型：
--
作者：
Kler, Stanislav;Asor, Roi;Li, Chenglei;Ginsburg, Avi;Harries, Daniel;Oppenheim, Ariella;Zlotnick, Adam;Raviv, Uri
值得注意的是，均匀的病毒样颗粒在一个似乎遵循快速动力学机制的过程中自组装。然而，球形病毒由核酸周围的数百种衣壳蛋白组装而成的机制尚不清楚。利用时间分辨小角度x射线散射（TR-SAXS），我们已经能够直接可视化封装短RNA分子（500米）的SV40 VP1五聚体。该组装过程产生由12个五聚体和1个RNA分子组成的T = 1个二十面体粒子。反应在35毫秒内完成了近1/3，遵循两态动力学过程，没有检测到中间产物。动力学理论分析表明，装配过程在RNA上成核，并通过一系列延伸反应继续进行，其中每次添加一个VP1五聚体，速率约为109 M−1 s−1。该反应具有很强的鲁棒性，并且比预测的扩散极限更快。新出现的分子机制似乎是病毒在核酸周围聚集的一般机制，暗示了长期静电相互作用。该模型提出，生长的核蛋白复合物充当静电天线，吸引其他衣壳亚基进行衣壳化过程。
Remarkably, uniform virus-like particles self-assemble in a process that appears to follow a rapid kinetic mechanism. The mechanisms by which spherical viruses assemble from hundreds of capsid proteins around nucleic acid, however, are yet unresolved. Using Time-Resolved Small-Angle X-ray Scattering (TR-SAXS) we have been able to directly visualize SV40 VP1 pentamers encapsidating short RNA molecules (500 mers). This assembly process yields T = 1 icosahedral particles comprised of 12 pentamers and one RNA molecule. The reaction is nearly 1/3 complete within 35 milliseconds, following a two–state kinetic process with no detectable intermediates. Theoretical analysis of kinetics, using a master equation, shows that the assembly process nucleates at the RNA and continues by a cascade of elongation reactions in which one VP1 pentamer is added at a time, with a rate of approximately 109 M−1 s−1. The reaction is highly robust and faster than the predicted diffusion limit. The emerging molecular mechanism, which appears to be general to viruses that assemble around nucleic acids, implicates long-ranged electrostatic interactions. The model proposes that the growing nucleo-protein complex acts as an electrostatic antenna that attracts other capsid subunits for the encapsidation process.
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影响因子：
2.9
作者：
Ceres, P;Zlotnick, A
通讯作者：
Zlotnick, A
影响因子：
64.8
作者：
LIDDINGTON, RC;YAN, Y;HARRISON, SC
通讯作者：
HARRISON, SC
影响因子：
3.7
作者：
Mukherjee S;Abd-El-Latif M;Bronstein M;Ben-nun-Shaul O;Kler S;Oppenheim A
通讯作者：
Oppenheim A
影响因子：
5.4
作者：
Li, PP;Nakanishi, A;Kasamatsu, H
通讯作者：
Kasamatsu, H
影响因子：
3.4
作者：
Moisant, Paul;Neeman, Henry;Zlotnick, Adam
通讯作者：
Zlotnick, Adam