Molecular genetic study on the crude herb Baizhi and its source
Molecular genetic study on the crude herb Baizhi and its source
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发表时间：
2004
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通讯作者：
Huang Lu-qi
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作者：
Huang Lu-qi
目的研究白芷药材来源之间的关系。方法选取七白芷、玉白芷、杭白芷、川白芷4个商品药材品种及其近缘药材，白芷（Angelica dahuica（菲施. Hoffm公司的）Benth et Hook.f.ex Franch.et Sav.、白花野牡丹A. formosana de Boiss、台湾拟步行虫A. pyrroplasticum Nakai et Kitagawa，Aamurensis pyrchk，A . baizhioides（Mss.），用40个随机引物对100株野生稻进行RAPD分析。结果共扩增出206个位点，其中多态性位点188个，多态性位点占88.3%，并建立了严格的一致性树。ITS 1序列为439bp，ITS 2序列为410bp。福尔摩沙。A. dahurica。建议将其命名为A.formosana de Boiss cv.药用植物十一月。台湾野牡丹、台湾野牡丹A.和白芷A.应将紫球藻作为不同的种来处理，并相互独立。
OBJECTIVE To study the relationship among the different sources of the crude herbs Baizhi. METHODS Four commodity varieties of crude herbs Qibaizhi, Yubaizhi, Hangbaizhi, Chuanbaizhi and their affinities, Angelica dahuica(Fisch. ex Hoffm. ) Benth et Hook.f.ex Franch.et Sav. ,A. formosana de Boiss, A. porphyrocaulis Nakai et Kitagawa,Aamurensis Schischk, A . baizhioides (Mss.), were characterized by RAPD method using 40 random primers. Based on the RAPD analysis, their ITS were sequenced and analysed.R ESULTS 206 loci were amplified, 188 loci of which were polymorphic(88.3% ) , and the strict consensus tree was concluded. ITS 1 sequence was 439 bp,and ITS 2 was 410 bp.CONCLUSION The four commodity varieties were originated from A. formosana. A. dahurica. They were suggested to be named as A.formosana de Boiss cv. officinarum cv. nov. . A .formosana, A. dahurica and A. porphyrocaulis should be treated as different species and independent from each other.