POSSIBLE ROLE OF RAT FATTY ACID-BINDING PROTEINS IN THE INTESTINE AS CARRIERS OF PHENOL AND PHTHALATE DERIVATIVES
POSSIBLE ROLE OF RAT FATTY ACID-BINDING PROTEINS IN THE INTESTINE AS CARRIERS OF PHENOL AND PHTHALATE DERIVATIVES
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DOI：
10.1016/0006-291x(90)91136-g
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发表时间：
1990-05-16
影响因子：
3.1
通讯作者：
MUTO, T
中科院分区：
文献类型：
--
作者：
KANDA, T;ONO, T;MUTO, T
采用Sephadex G-75凝胶过滤和deae -纤维素分离纯化大鼠肠细胞质中肝、肠型脂肪酸结合蛋白和胃促生成素样蛋白（GTLP）。cm -纤维素和羟基磷灰石色谱。除脂肪酸外，通过气相液相色谱和质谱分析，从脂肪酸结合蛋白超家族的提取物中鉴定出丁基羟基甲苯（BHT）、邻苯二甲酸二丁基酯和二（2-乙基己基）酯（DBP和DEHP）为内源性配体。肠道中的这些蛋白家族可能在阻止这些外源污染物的初始步骤中作为载体发挥重要作用。
Fatty acid-binding proteins of hepatic and intestinal type and gastrotropin-like protein (GTLP) were purified from rat intestinal cytosol by Sephadex G-75 gel filtration and DEAE-cellulose. CM-cellulose, and hydroxylapatite chromatographies. In addition to fatty acids, butylated hydroxytoluene (BHT), phthalate dibutyl, and di(2-ethylhexyl) esters (DBP and DEHP) were identified by gas liquid chromatography and mass spectrometry as endogenous ligands from the extract of either fatty acid-binding protein superfamily. These protein families in the intestine may have an important role as carriers in the initial step of arresting these exogenous pollutants.