Two-photon fluorescence endoscopy with a micro-optic scanning head
Two-photon fluorescence endoscopy with a micro-optic scanning head
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DOI：
10.1364/ol.28.001552
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发表时间：
2003-09-01
期刊：
影响因子：
3.6
通讯作者：
Gu, M
中科院分区：
文献类型：
--
作者：
Bird, D;Gu, M
在开发双光子荧光内窥镜的竞赛中的一个主要障碍是使用复杂的大体积光学器件来传输超短脉冲激光束并返回发射的荧光信号。本文介绍了一种基于单模光纤耦合器、微棱镜和梯度折射率棒透镜的全光纤双光子荧光显微内窥镜。结果发现，新的内窥镜具有3.2微米的轴向分辨率，并能够成像的内部圆柱形结构的横截面小到约3.0毫米的直径。该设备展示了开发用于生物医学研究的实时诊断工具而无需手术活检的潜力，并可能在光动力治疗，显微外科手术和早期癌症检测中找到应用。(C)2003年美国光学学会。
A major obstacle in the race to develop two-photon fluorescence endoscopy is the use of complicated bulk optics to transmit an ultrashort-pulsed laser beam and return the emitted fluorescence signal. We describe an all-fiber two-photon fluorescence microendoscope based on a single-mode optical fiber coupler, a microprism, and a gradient-index rod lens. It is found that the new endoscope exhibits an axial resolution of 3.2 mum and is capable of imaging transverse cross sections of internal cylindrical structures as small as approximately 3.0 mm in diameter. This device demonstrates the potential for developing a real-time diagnostic tool for biomedical research without the need for surgical biopsy and may find applications in photodynamic therapy, microsurgery, and early cancer detection. (C) 2003 Optical Society of America.