Lipid profile perturbations in the plasma and lungs of mice with LPS-induced acute lung injury revealed by UHPLC-ESI-Q Exactive HF MS analysis
Lipid profile perturbations in the plasma and lungs of mice with LPS-induced acute lung injury revealed by UHPLC-ESI-Q Exactive HF MS analysis
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UHPLC-ESI-Q Exactive HF MS 分析揭示 LPS 诱导的急性肺损伤小鼠血浆和肺部的脂质谱扰动
DOI：
10.1016/j.jpba.2018.09.037
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发表时间：
2019-01-05
影响因子：
3.4
通讯作者：
Zhou, Wei
中科院分区：
文献类型：
--
作者：
Shan, Jinjun;Qian, Wenjuan;Zhou, Wei
基于UHPLC-ESI-Q Exactive HF MS的脂质组学方法成功应用于分析脂多糖（LPS）鼻内激发小鼠血浆和肺中的各种脂质。脂质对LPS的响应趋势通过变量重要性投影（VIP）图、热图和条形图以图形方式表示。结果，通过比较健康小鼠和LPS诱导的小鼠，确定了肺中的77种差异脂质和血浆中的13种差异脂质。这些结果揭示了炎症与脂质代谢之间的相关性。差异调节的脂质也可能用作炎症的生物标志物。(C)2018 Elsevier B. V.版权所有。
An UHPLC-ESI-Q Exactive HF MS-based lipidomics method was successfully applied to profile various lipids from the plasma and lungs of mice intranasally challenged with lipopolysaccaride (LPS). Response trends of lipids to LPS were graphically represented by variable importance in projection (VIP) plot, heat map, and bar plot. As a result, 77 differential lipids in the lung and 13 differential lipids in the plasma were identified by comparison between healthy and LPS-induced mice. These results revealed the correlation between inflammation and lipids metabolism. The differentially regulated lipids could also be potentially used as biomarkers for inflammation. (C) 2018 Elsevier B.V. All rights reserved.