LIMITED HETEROGENEITY OF REARRANGED T-CELL RECEPTOR V-ALPHA TRANSCRIPTS IN BRAINS OF MULTIPLE-SCLEROSIS PATIENTS
LIMITED HETEROGENEITY OF REARRANGED T-CELL RECEPTOR V-ALPHA TRANSCRIPTS IN BRAINS OF MULTIPLE-SCLEROSIS PATIENTS
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DOI：
10.1038/345344a0
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发表时间：
1990-05-24
期刊：
影响因子：
64.8
通讯作者：
BERNARD, CCA
中科院分区：
文献类型：
--
作者：
OKSENBERG, JR;STUART, S;BERNARD, CCA
激活的识别？多发性硬化症（MS）患者大脑中的细胞表明，这些细胞在这种疾病的发病机制中起着关键作用。为了阐明淋巴细胞浸润的性质，我们使用聚合酶链反应从MS脑病变的转录本中扩增t细胞抗原受体（TCR） v α序列。在三个多发性硬化症大脑中，每个大脑只检测到2到4个重排的TCR v α转录物。在对照脑中未发现v α转录本。对Vα12.1区编码的转录本进行序列分析，发现有少量的j α区片段发生重排。提示TCR v α基因在MS脑病变中的表达受到限制。
THE identification of activated ? cells in the brain of individuals with multiple sclerosis (MS) indicates that these cells are critical in the pathogenesis of this disease. In an attempt to elucidate the nature of the lymphocytic infiltration, we used the polymerase chain reaction to amplify T-cell antigen receptor (TCR) Vαsequences from transcripts derived from MS brain lesions. In each of three MS brains, only two to four rearranged TCR Vαtranscripts were detected. No Vαtranscripts could be found in control brains. Sequence analysis of transcripts encoded by the Vα12.1 region showed rearrangements to a limited number of Jαregion segments. These results imply that TCR Vαgene expression in MS brain lesions is restricted.